MicroRNA-214 Promotes Apoptosis in Canine Hemangiosarcoma by Targeting the COP1-p53 Axis

PloS One
Kazuki HeishimaKohji Maruo

Abstract

MicroRNA-214 regulates both angiogenic function in endothelial cells and apoptosis in various cancers. However, the regulation and function of miR-214 is unclear in canine hemangiosarcoma, which is a spontaneous model of human angiosarcoma. The expression and functional roles of miR-214 in canine hemangiosarcoma were presently explored by performing miRNA TaqMan qRT-PCR and transfecting cells with synthetic microRNA. Here, we report that miR-214 was significantly down-regulated in the cell lines used and in clinical samples of canine hemangiosarcoma. Restoration of miR-214 expression reduced cell growth and induced apoptosis in canine hemangiosarcoma cell lines through transcriptional activation of p53-regulated genes although miR-214 had a slight effect of growth inhibition on normal endothelial cells. We identified COP1, which is a critical negative regulator of p53, as a novel direct target of miR-214. COP1 was overexpressed and the specific COP1 knockdown induced apoptosis through transcriptional activation of p53-regulated genes as well as did miR-214-transfection in HSA cell lines. Furthermore, p53 knockdown abolished the miR-214-COP1-mediated apoptosis; thus, miR-214 and COP1 regulated apoptosis through controlling p53 i...Continue Reading

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Citations

Oct 30, 2015·International Journal of Molecular Sciences·Kazuki HeishimaKohji Maruo
Jan 10, 2018·Veterinary and Comparative Oncology·Y-C LaiN Miura
Apr 26, 2016·Oncology Reports·Bo LiuLike Zhang
May 26, 2017·Scientific Reports·Kazuki HeishimaTakashi Mori
Oct 4, 2020·Veterinary Sciences·Olivia Gourbault, Lola Llobat
Sep 21, 2017·Journal of Veterinary Science·Kabiru SahabiGeok Chin Tan
May 25, 2016·Journal of Cellular Biochemistry·Juan LiuZhaohui Feng
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May 2, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Xiaoxin Wang, Ying Song

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Methods Mentioned

BETA
ubiquitination
biopsy
transfection
electrophoresis
Assay
protein
PCR

Software Mentioned

TargetScan

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