MicroRNA-214 suppresses the proliferation of human hepatocellular carcinoma cells by targeting E2F3

Oncology Letters
Yang YangChen Huang

Abstract

MicroRNAs (miRNAs) are important gene regulators that participate in tumorigenesis. Previous studies have implicated that miR-214 is a tumor suppressor that is capable of inhibiting human hepatocellular carcinoma (HCC) cell growth. However, the mechanism by which miR-214 suppresses tumor development remains unknown. In the present study, miR-214 was observed to suppress tumor proliferation by directly targeting E2F transcription factor 3 (E2F3) in HCC cells. Colony formation, cell cycle and proliferation assays were employed to study the tumor suppressor role of miR-214 in cell proliferation. In addition, western blotting and dual-luciferase reporter assays were used to evaluate whether E2F3 was a target of miR-214. The results of these analyses revealed that E2F3 was a novel target of miR-214. Furthermore, enhanced expression of miR-214 or silencing of E2F3 inhibited the proliferation of HCC SMMC-7721 cells. These findings suggest that miR-214 suppresses HCC growth by targeting E2F3, and may provide a novel approach for the treatment of human HCC.

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Citations

Nov 4, 2017·American Journal of Physiology. Gastrointestinal and Liver Physiology·Xinli HuangSen Lu
May 16, 2018·Journal of Cellular and Molecular Medicine·Meixiao ZhanLigong Lu
Aug 12, 2017·Cancer Biomarkers : Section a of Disease Markers·Xun GouZhengrong Wang
Aug 26, 2016·Endocrine Regulations·K VoglovaIveta Herichova
Aug 26, 2016·Endocrine Regulations·K VoglovaIveta Herichova

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