MicroRNA-216a inhibits neuronal apoptosis in a cellular Parkinson's disease model by targeting Bax.

Metabolic Brain Disease
Xiaobo YangHongmei Cao

Abstract

The study found that microRNAs play an important role in Parkinson's disease (PD). However, the function of MicroRNA-216a (miR-216a) in PD is unclear. Therefore, this experiment aimed to investigate the pathogenesis of miR-216a in PD. Using the toxicity of MPP+ to polyhexamine neurons, apoptosis of SH-SY5Y neuroblastoma cells was induced at different time by MPP+ to construct a stable acute PD cell model. The effects of DNA breakage, mitochondrial membrane potential (A ^ m), caspase-3 activity and nucleosome enrichment on cell apoptosis were detected by flow cytometry, TUNEL. MPP+ increased the toxic effects of dopaminergic neurons in a PD model. The introduction of miR-216a inhibited MPP + -induced neuronal apoptosis. The main manifestations were the decreased levels of positive rate of Tunel cells, caspase 3 activity and nucleosome enrichment factor. Bax was a direct target of miR-216a. In addition, Bax overexpression reversed the effects of miR-216a on neural cells. Bax downstream factors were also involved in miR-216a regulation of MPP + -triggered neuronal apoptosis. miR-216a regulated the progression of PD by regulating Bax, and miR-216a may be a potential target for PD.

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Citations

Jul 8, 2020·Antioxidants·Han-A Park, Amy C Ellis
Feb 26, 2021·Neuropsychiatric Disease and Treatment·Xudong QianJian Zhang
May 18, 2021·Frontiers in Neuroscience·Yong Hui NiesSeong Lin Teoh

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Methods Mentioned

BETA
transfection
PCR
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SPSS
Quantity One

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis