MicroRNA-218-5p inhibit the migration and proliferation of pterygium epithelial cells by targeting EGFR via PI3K/Akt/mTOR signaling pathway

Experimental Eye Research
Shuang HanYichun Kong

Abstract

Pterygium is a common ocular surface disease which could result in various ocular surface symptoms. MicroRNAs play an important role in the development of various eye diseases. However, the role of microRNAs in the pathogenesis of pterygium is rarely reported. Our research aims to analyze the relationship between miR-218-5p and Epidermal Growth Factor Receptor (EGFR) in human pterygium tissues and cultured Human Pterygium Epithelial Cells (hPECs). Furthermore, the EGFR/PI3K/Akt/mTOR signaling pathway was firstly verified in pterygium. Pterygium tissues and normal bulbar conjunctival tissues were obtained from surgery, and primary hPECs were cultured in vitro. Cell transfection, Quantitative real-time PCR (qRT-PCR), Western blotting, Luciferase reporter assay and Scratch Wound Healing Assay were performed. Our data demonstrated that miR-218-5p was decreased and EGFR was increased in pterygium tissues than normal conjunctival tissues. In transfected hPECs, our results indicated that upregulated miR-218-5p significantly suppressed the expression level of EGFR via PI3K/Akt/mTOR pathway. In addition, the migration and proliferation of hPECs was promoted by miR-218-5p inhibitor and retarded by miR-218-5p mimics. And knockdown of EGFR...Continue Reading

Citations

Mar 26, 2019·Investigative Ophthalmology & Visual Science·Kailin ChenKe Yao
Jul 10, 2020·Investigative Ophthalmology & Visual Science·Nuo XuLi Huang
Jul 26, 2019·BioMed Research International·Siying HeGuohua Yang
Mar 9, 2019·Journal of Cellular Physiology·Seyed Elyas MeshkaniAbolfazl Rad
Oct 2, 2020·Applied Immunohistochemistry & Molecular Morphology : AIMM·Si WangYang Liu
May 18, 2020·International Immunopharmacology·Gholamreza Rezaei DanbaranHamed Mohammadi
Dec 11, 2020·BioMed Research International·Yuting XuFang Zheng
Aug 10, 2021·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elayaraja KolanthaiSudipta Seal
Jan 9, 2022·Scientific Reports·Juliana Albano de GuimarãesMonica Alves

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