MicroRNA-22 Promotes Renal Tubulointerstitial Fibrosis by Targeting PTEN and Suppressing Autophagy in Diabetic Nephropathy

Journal of Diabetes Research
YingYing ZhangBing Guo

Abstract

Renal tubulointerstitial fibrosis (TIF) is a major feature of diabetic nephropathy (DN). There is increasing evidence demonstrating that microRNAs act as key players in the regulation of autophagy and are involved in DN. However, the exact link among microRNAs, autophagy, and TIF in DN is largely unknown. In this study, our results showed that TIF was observed in DN rats together with obvious autophagy suppression. Moreover, microRNA-22 (miR-22) was upregulated and associated with reduced expression of its target gene phosphatase and tensin homolog (PTEN) in both the kidneys of DN rats and high glucose-cultured NRK-52E cells. Intriguingly, induction of autophagy by rapamycin antagonized high glucose-induced collagen IV (Col IV) and α-SMA expression. In addition, ectopic expression of miR-22 suppressed autophagic flux and induced the expression of Col IV and α-SMA, whereas the inhibition of endogenous miR-22 effectively relieved high glucose-induced autophagy suppression and the expression of Col IV and α-SMA in NRK-52E cells. Overexpression of PTEN protectively antagonized high glucose- and miR-22-induced autophagy suppression and the expression of Col IV. Therefore, our findings indicated that miR-22 may promote TIF by suppres...Continue Reading

References

Aug 16, 2002·Medical Electron Microscopy : Official Journal of the Clinical Electron Microscopy Society of Japan·Keisuke InaTatsuo Shimada
Feb 6, 2013·PloS One·Dagmar KleinRicardo L Pastori
Sep 27, 2014·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·Katherine R TuttleMark E Molitch
Sep 27, 2014·Journal of Diabetes Research·Hao WuLining Miao
Jun 27, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Dong JiGuofeng Chen
Sep 30, 2015·Clinical Science·Aaron D McClellandMark E Cooper
Oct 9, 2015·Journal of Cellular Biochemistry·Lin ZhuJun Hao
Dec 25, 2015·Journal of the American Society of Nephrology : JASN·Xiaoxu ZhouShougang Zhuang
Jan 28, 2017·Molecular Therapy : the Journal of the American Society of Gene Therapy·Malte KöllingJohan M Lorenzen
Mar 9, 2017·The American Journal of Pathology·Shwetha K ShettySreerama Shetty
Apr 26, 2017·Autophagy·Jian ZhangDa Pang
Jul 19, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Aysa RezabakhshAlireza Garjani

❮ Previous
Next ❯

Citations

Dec 13, 2018·Physiology·Jeffrey C Horowitz, Victor J Thannickal
Mar 26, 2019·Acta Biochimica Et Biophysica Sinica·Xiaomin LiuYanyun He
Jan 26, 2020·International Journal of Molecular Sciences·Kenji TsujiJun Wada
May 16, 2020·Bioscience Reports·Xudong WangJianping Yang
Nov 21, 2019·International Journal of Molecular Medicine·Zhen-Huan JiangChang-Lin Ni
Nov 22, 2018·Disease Models & Mechanisms·Carmen Hurtado Del PozoNuria Montserrat
Jan 16, 2020·Biomolecules·Gur P KaushalSudhir V Shah
Mar 21, 2020·Frontiers in Pharmacology·Tamil Selvi LoganathanNoraidatulakma Abdullah
Sep 11, 2020·International Journal of Molecular Sciences·Linsey J F PetersEmiel P C van der Vorst
Dec 14, 2018·Frontiers in Veterinary Science·Osamu IchiiYasuhiro Kon
Apr 26, 2019·International Journal of Molecular Sciences·Cynthia Van der HauwaertChristelle Cauffiez
Jan 18, 2019·Journal of Cellular Biochemistry·Rui YanBing Guo
Apr 21, 2020·Critical Reviews in Clinical Laboratory Sciences·Manoj KhokharPraveen Sharma
May 18, 2020·International Immunopharmacology·Gholamreza Rezaei DanbaranHamed Mohammadi
Feb 13, 2021·Frontiers in Pharmacology·Yue-Yu GuHui-Yao Lan
Feb 1, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Yunus Akkoc, Devrim Gozuacik
Feb 28, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Junying LuShu Nie
Jun 26, 2018·Biochemical Pharmacology·Saikat Dewanjee, Niloy Bhattacharjee
Apr 17, 2021·Frontiers in Medicine·Jingsheng ChangYifei Zhong
Apr 20, 2021·Frontiers in Cell and Developmental Biology·Runze WangMingming Zhang
Nov 19, 2020·Biochemical Pharmacology·Amir YarahmadiNegar Azarpira
Aug 23, 2021·Pathology, Research and Practice·Hui ZhaoJun Yu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transfection
confocal microscopy

Software Mentioned

miRanda
Image
ImageJ
Pro
TargetScan

Related Concepts

Related Feeds

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms