MicroRNA-221/222 confers tamoxifen resistance in breast cancer by targeting p27Kip1.

The Journal of Biological Chemistry
Tyler E MillerSarmila Majumder

Abstract

We explored the role of microRNAs (miRNAs) in acquiring resistance to tamoxifen, a drug successfully used to treat women with estrogen receptor-positive breast cancer. miRNA microarray analysis of MCF-7 cell lines that are either sensitive (parental) or resistant (4-hydroxytamoxifen-resistant (OHT(R))) to tamoxifen showed significant (>1.8-fold) up-regulation of eight miRNAs and marked down-regulation (>50%) of seven miRNAs in OHT(R) cells compared with parental MCF-7 cells. Increased expression of three of the most promising up-regulated (miR-221, miR-222, and miR-181) and down-regulated (miR-21, miR-342, and miR-489) miRNAs was validated by real-time reverse transcription-PCR. The expression of miR-221 and miR-222 was also significantly (2-fold) elevated in HER2/neu-positive primary human breast cancer tissues that are known to be resistant to endocrine therapy compared with HER2/neu-negative tissue samples. Ectopic expression of miR-221/222 rendered the parental MCF-7 cells resistant to tamoxifen. The protein level of the cell cycle inhibitor p27(Kip1), a known target of miR-221/222, was reduced by 50% in OHT(R) cells and by 28-50% in miR-221/222-overexpressing MCF-7 cells. Furthermore, overexpression of p27(Kip1) in the res...Continue Reading

References

Oct 1, 1996·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C CarlomagnoS De Placido
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 3, 2002·Nature Medicine·Stacy W Blain, Joan Massagué
Apr 10, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elton R KisangaErnst A Lien
Jun 17, 2004·Journal of the National Cancer Institute·Jiang ShouRachel Schiff
Jul 23, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Li ZhuMazakazu Toi
Jul 26, 2005·Biochemical and Biophysical Research Communications·S A CiafrèM G Farace
Aug 17, 2005·Cancer Research·Marilena V IorioCarlo M Croce
May 27, 2006·Endocrine-related Cancer·P PallanteA Fusco
Dec 7, 2006·International Journal of Cancer. Journal International Du Cancer·Eun Joo LeeThomas D Schmittgen
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Feb 1, 2008·The New England Journal of Medicine·Carlo M Croce
Apr 17, 2008·Cancer Research·Ricardo MedinaGary S Stein

❮ Previous
Next ❯

Citations

Aug 29, 2012·Cellular and Molecular Life Sciences : CMLS·Huiping Liu
Sep 1, 2012·Cellular and Molecular Life Sciences : CMLS·Min Xu, Yin-Yuan Mo
Jul 9, 2010·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Hiroshi I Suzuki, Kohei Miyazono
Jun 10, 2009·Breast Cancer Research and Treatment·Marc P J McCaffertyMichael J Kerin
Sep 4, 2009·Breast Cancer Research and Treatment·Lauren A LicataJudith Clancy Keen
Jan 8, 2010·Breast Cancer Research and Treatment·Selma MasriShiuan Chen
Mar 15, 2011·Breast Cancer Research and Treatment·L KastlA C Schofield
Jul 15, 2011·Breast Cancer Research and Treatment·Nobuyasu YoshimotoHiroko Yamashita
May 26, 2010·Journal of Mammary Gland Biology and Neoplasia·Josephine A WrightGregory J Goodall
Feb 22, 2012·Journal of Mammary Gland Biology and Neoplasia·Erin N HoweJennifer K Richer
Mar 3, 2012·Journal of Mammary Gland Biology and Neoplasia·Zuoren Yu, Richard G Pestell
Aug 24, 2012·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Wei TianYongchuan Deng
Jul 16, 2011·Hormones & Cancer·Dawn R CochraneJennifer K Richer
Mar 27, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Wei-Xian ChenJian-Hua Zhao
Jun 7, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Zhu JianweiLi Can
Nov 2, 2011·The Breast : Official Journal of the European Society of Mastology·Marilena V IorioElda Tagliabue
Feb 22, 2012·Expert Reviews in Molecular Medicine·Aliaa M SeoudiAhmed I Abdelaziz
May 15, 2010·Cancer Gene Therapy·J MaC Ji
Aug 25, 2009·Nature Reviews. Cancer·Elizabeth A Musgrove, Robert L Sutherland
Aug 10, 2012·Molecular BioSystems·Xing ChenGui-Ying Yan
Jun 16, 2010·Proceedings of the National Academy of Sciences of the United States of America·Swadesh K DasPaul B Fisher
Nov 18, 2009·The Journal of Infectious Diseases·Ai-Yu GongXian-Ming Chen
Jul 12, 2013·Cancer Biotherapy & Radiopharmaceuticals·Banzhou PanHaizhu Song
Apr 12, 2012·Carcinogenesis·Marilena V Iorio, Carlo M Croce
Apr 15, 2010·Journal of the National Cancer Institute·Gianpiero Di LevaCarlo M Croce
Mar 7, 2009·Nucleic Acids Research·Nalinie S WickramasingheCarolyn M Klinge
Aug 27, 2010·Current Opinion in Oncology·Michael A TesselSteven T Rosen
Jul 6, 2010·Otology & Neurotology : Official Publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology·David J ArchibaldEugene D Kwon
Jun 1, 2012·The Cancer Journal·Marilena V Iorio, Carlo M Croce
Jun 1, 2012·The Cancer Journal·Marco GalassoStefano Volinia

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.