MicroRNA-223 Regulates Retinal Function and Inflammation in the Healthy and Degenerating Retina

Frontiers in Cell and Developmental Biology
Nilisha FernandoRiccardo Natoli

Abstract

MicroRNAs (miRNAs) are small, non-coding RNA molecules that have powerful regulatory properties, with the ability to regulate multiple messenger RNAs (mRNAs) and biological pathways. MicroRNA-223-3p (miR-223) is known to be a critical regulator of the innate immune response, and its dysregulation is thought to play a role in inflammatory disease progression. Despite miR-223 upregulation in numerous neurodegenerative conditions, largely in cells of the myeloid lineage, the role of miR-223 in the retina is relatively unexplored. Here, we investigated miR-223 in the healthy retina and in response to retinal degeneration. miR-223-null mice were investigated in control and photo-oxidative damage-induced degeneration conditions. Encapsulated miR-223 mimics were intravitreally and intravenously injected into C57BL/6J wild-type mice. Retinal functional responses were measured using electroretinography (ERG), while extracted retinas were investigated by retinal histology (TUNEL and immunohistochemistry) and molecular analysis (qPCR and FACS). Retinal function in miR-223-/- mice was adversely affected, indicating that miR-223 may be critical in regulating the retinal response. In degeneration, miR-223 was elevated in the retina, circulat...Continue Reading

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Citations

Oct 8, 2020·Cells·Marija PetkovicLouise Torp Dalgaard
Feb 2, 2021·Frontiers in Cell and Developmental Biology·Daniela IntartagliaIvan Conte

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Methods Mentioned

BETA
fluorescence-activated cell sorting
mechanical dissociation
FACS
transfection

Software Mentioned

ImageJ
Prism
ZEISS
GraphPad
Espion

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