MicroRNA-24-1 suppresses mouse hepatoma cell invasion and metastasis via directly targeting O-GlcNAc transferase

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Yubo LiuJianing Zhang

Abstract

MicroRNAs (miRNAs) are endogenous non-coding regulatory RNAs involved in multiple cellular processes. Emerging evidences showed that miRNAs are involved in changing the cell surface glycosylation modification and oncogenesis. In this study, the role of miRNA-24-1 in O-GlcNAcylation and metastasis of mouse hepatocarcinoma cells was investigated. miRNAs expression array profiles were obtained from mouse hepatocarcinoma cell lines Hca-P and Hca-F with the low/high lymphatic metastasis potential, respectively. Based on the miRNAs expression array profiles, miRNA-24-1 expression was found to exhibit converse coincidence with metastasis potential, O-GlcNAc transferase (OGT) expression and O-GlcNAcylation. Dual-luciferase reporter assay revealed that miRNA-24-1 specifically binds to 3'-UTR of OGT. Furthermore, transfecting mouse hepatocarcinoma cells with miR-24-1 mimic and antisense oligonucleotide showed miR-24-mediates OGT expression silencing. This silencing is associated with the suppression of cell metastasis potential, down-regulation of the O-GlcNAcylation on c-Myc and decrease of c-Myc expression at the protein level rather than the mRNA level. Collectively, these results suggested that as a tumor suppressor, miR-24-1 may reg...Continue Reading

Citations

Mar 30, 2018·Journal of Bioenergetics and Biomembranes·John A HanoverMichelle R Bond
Nov 20, 2018·Molecular Oncology·Lotteke J Y M SwierJoost Kluiver
Nov 17, 2020·Frontiers in Oncology·Donglu WuHaoming Luo
Dec 11, 2020·BioMed Research International·Mohamed H YousefAnwar Abdelnaser
Jul 31, 2020·Physiological Reviews·John C ChathamAdam R Wende
Apr 4, 2021·International Journal of Molecular Sciences·Rossella Indellicato, Marco Trinchera
Oct 6, 2019·Biochemistry·Chu T Thu, Lara K Mahal
Aug 28, 2021·Biomedicines·Ekaterina Mikhailovna StasevichAnton Markovich Schwartz

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