MicroRNA-25 Negatively Regulates Cerebral Ischemia/Reperfusion Injury-Induced Cell Apoptosis Through Fas/FasL Pathway

Journal of Molecular Neuroscience : MN
Jun-Feng ZhangYing-Fang Tian

Abstract

MicroRNA-25 (miR-25) has been reported to be a major miRNA marker in neural cells and is strongly expressed in ischemic brain tissues. However, the precise mechanism and effect of miR-25 in cerebral ischemia/reperfusion (I/R) injury needs further investigations. In the present study, the oxygen-glucose deprivation (OGD) model was constructed in human SH-SY5Y and IMR-32 cells to mimic I/R injury and to evaluate the role of miR-25 in regulating OGD/reperfusion (OGDR)-induced cell apoptosis. We found that miR-25 was downregulated in the OGDR model. Overexpression of miR-25 via miRNA-mimics transfection remarkably inhibited OGDR-induced cell apoptosis. Moreover, Fas was predicted as a target gene of miR-25 through bioinformatic analysis. The interaction between miR-25 and 3'-untranslated region (UTR) of Fas mRNA was confirmed by dual-luciferase reporter assay. Fas protein expression was downregulated by miR-25 overexpression in OGDR model. Subsequently, the small interfering RNA (siRNA)-mediated knockdown of Fas expression also inhibited cell apoptosis induced by OGDR model; in contrast, Fas overexpression abrogated the protective effects of miR-25 on OGDR-induced cells. Taken together, our results indicate that the upregulation of...Continue Reading

References

Apr 1, 1997·Seminars in Immunology·P A HenkartA Sarin
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Sep 15, 2007·The New England Journal of Medicine·Derek M Yellon, Derek J Hausenloy
Feb 9, 2008·Stroke; a Journal of Cerebral Circulation·Kandiah JeyaseelanArunmozhiarasi Armugam
Apr 22, 2008·Nature Immunology·Eric VivierSophie Ugolini
Jan 10, 2009·Results and Problems in Cell Differentiation·Madhu RamaswamyRichard M Siegel
Mar 4, 2009·Nature Cell Biology·Julia WinterSven Diederichs
Jun 3, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Heng-Chao YuHua Han
Mar 20, 2012·Cell·Joshua T Mendell, Eric N Olson
Jan 4, 2013·Circulation·Alan S GoUNKNOWN American Heart Association Statistics Committee and Stroke Statistics Subcommittee
Oct 1, 2013·Molecular and Cellular Biochemistry·Hongying ZhaoWenqing Li
Oct 16, 2014·Journal of Molecular Neuroscience : MN·Weidong HuangJie Zhang
Jan 22, 2015·Stroke; a Journal of Cerebral Circulation·Creed M StaryRona G Giffard
Mar 13, 2015·International Journal of Molecular Sciences·Lei PanYi-Han Chen
Jul 26, 2015·Molecular and Cellular Biochemistry·Gang PengWeixi Jiang
Sep 5, 2015·Mediators of Inflammation·Samuel García, Carmen Conde
Sep 8, 2015·Nature Cell Biology·Hui-Chen ChenEmily H Cheng
Sep 9, 2015·Cellular and Molecular Neurobiology·Esmat AmiriMaryam Moosavi

❮ Previous
Next ❯

Citations

Aug 30, 2016·Biochemical and Biophysical Research Communications·Wanqian LiangYuan Dong
Mar 24, 2016·Scientific Reports·Zhi-Yu DuanXiang-Mei Chen
May 24, 2017·Journal of Neuropathology and Experimental Neurology·Seyed Esmaeil KhoshnamMaryam Farzaneh
May 10, 2017·Journal of Stroke·Seyed Esmaeil KhoshnamMaryam Farzaneh
Jun 14, 2017·The Journal of Spinal Cord Medicine·Yi Guo, Shizhen Niu
Nov 8, 2018·Médecine sciences : M/S·Qun-Qun Jiang, Wei-Bing Liu
Feb 5, 2019·CNS & Neurological Disorders Drug Targets·Fatemeh ForouzanfarElham Amini
Aug 31, 2016·Molecular Medicine Reports·Zhiyou CaiGuanghui Chen
Jul 2, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Mingjuan CaoDingbo Tao
May 17, 2018·Oncotarget·Márta SárközyTamás Csont
Dec 26, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Peijun Tian, Li Yan
Apr 4, 2019·Molecular Medicine Reports·Dapeng LinGuanjun Tu
Feb 25, 2020·BioMed Research International·Li ZhangJunfang Teng
Jun 25, 2020·Neurotoxicity Research·Meng LuSeyed Esmaeil Khoshnam
Oct 9, 2020·Immunological Investigations·Fatemeh Sabet Sarvestani, Negar Azarpira
Jan 8, 2021·Environmental Science and Pollution Research International·Shouxiang XuJuan Zhang
Dec 29, 2020·Cellular and Molecular Neurobiology·Rais Reskiawan A KadirUlvi Bayraktutan
Jun 22, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Soudeh Ghafouri-FardMohammad Taheri
May 8, 2021·Neuropsychiatric Disease and Treatment·Xiaoli RenCongfang Guo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.