MicroRNA-26a-CD36 signaling pathway: Pivotal role in lipid accumulation in hepatocytes induced by PM2.5 liposoluble extracts

Environmental Pollution
Dongxiao DingSijun Dong

Abstract

Exposure to ambient particular matters (PM) has been associated with the development of non-alcoholic fatty liver disease (NAFLD), but the underlying mechanism remains unclear. Given that microRNA (miRNA) is recognized as a key regulator of lipid metabolism and a potential mediator of environmental cues, this study aimed to explore the role of miRNA-mRNA regulation underlying abnormal lipid metabolism triggered by PM2.5liposoluble extracts. We confirmed that 72-h exposure to liposoluble extracts of PM2.5 from Nanjing at 25 μg/cm2 induced lipid accumulation in HepG2 cells by promoting uptake of free fatty acids (FFAs). Notably, lipid accumulation induced by PM2.5 liposoluble extracts was associated with decreased expression of miR-26a and consequent upregulation of fatty acid translocase (FAT, also known as CD36). Using gain- and loss-of-function assays, we demonstrated that miR-26a negatively regulated CD36 to mediate lipid accumulation in HepG2 cells. We further confirmed that miR-26a directly acted on the 3' untranslated region (3'UTR) of CD36. Furthermore, overexpression of miR-26a abolished steatosis in HepG2 cells treated with PM2.5 liposoluble extracts by suppressing CD36. In addition, we demonstrated that PM2.5 liposolub...Continue Reading

Citations

Jun 20, 2020·Environmental Science and Pollution Research International·Mengyao WangZhijun Huang
Oct 30, 2020·Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy·Qiuli ShanJing Guo
Jan 30, 2021·Life Sciences·Jiaxin ChenNingning Wang
Aug 27, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xiao-Fei PengWen-Chu Ye
Sep 27, 2020·Cell Death & Disease·Patricia RadaÁngela M Valverde

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