MicroRNA-26a overexpression protects RGC-5 cells against H2O2-induced apoptosis

Biochemical and Biophysical Research Communications
Ye KangXiangze Yang

Abstract

We intended to examine the functional role of microRNA 26 (miR-26a) in regulating H2O2-induced cytotoxicity and apoptosis in RGC-5 cells in vitro. Various concentrations of H2O2 (0-1000 μM) were added in RGC-5 culture. Cell cytotoxicity was monitored by viability assay and gene expression level of miR-26a examined by qRT-PCR. MicroRNA-26a mimic was then applied in the RGC-5 culture to examine its effect on upregulating endogenous miR-26a and rescuing H2O2-induced cytotoxicity. TUNEL immunostaining assay was used to further assess the protective effect of upregulating miR-26a on H2O2-induced apoptosis in RGC-5 cells. Direct targeting of miR-26a on Phosphatase and tensin homolog (PTEN) signaling pathway was assessed by luciferase assay and western blotting. PTEN was then ectopically over-expressed in RGC-5. And its effects on miR-26a mediated apoptosis protection in RGC-5 were investigated by western blot and TUNEL assay. H2O2 induced cytotoxicity and down-regulated miR-26a in dose-dependent manner in RGC-5 cells. MiR-26a-mimic upregulated endogenous miR-26a gene levels, and then reduced H2O2-induced cytotoxicity, as well as H2O2-induced apoptosis in RGC-5 cells. PTEN was directly targeted by miR-26a. PTEN protein was upregulated...Continue Reading

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Citations

May 29, 2015·International Journal of Molecular Sciences·Na ZhouHuanran Tan
Dec 30, 2017·International Journal of Molecular Medicine·Haiyan LiChaoyi Qu
Sep 16, 2017·Acta Biochimica Et Biophysica Sinica·Ying TianXiaobo Xia
Mar 7, 2021·International Journal of Molecular Sciences·Saray TabakElie Beit-Yannai

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