MicroRNA-26b inhibits metastasis of osteosarcoma via targeting CTGF and Smad1

Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine
Guoqing DuanShiqing Feng

Abstract

Downregulation of miR-26b has been found in various cancers, but it has never been investigated in osteosarcoma. In this study, we demonstrated downregulation of miR-26b in osteosarcoma tissues, negatively correlated with the expression of connective tissue growth factor (CTGF) and Smad1. Luciferase reporter assay confirmed the interaction of miR-26b with the 3' untranslated regions (UTRs) of CTGF and Smad1. Transfection of miR-26b in osteosarcoma cells suppressed the expression of CTGF and Smad1, suggesting CTGF and Smad1 as direct targets of miR-26b. Overexpression of miR-26b inhibited the migration of osteosarcoma cells, which was reversed by overexpression of CTGF or Smad1. Knockdown of CTGF by small interfering RNA (siRNA) interference blocked the activation of Smad1, ERK1/2, and MMP2, which was opposite to the overexpression of CTGF. Differently, Smad1 did not significantly affect CTGF level, but mediated ERK1/2 phosphorylation and MMP2 activation. Furthermore, miR-26b inhibited lung metastasis of osteosarcoma in vivo. Our data indicated that downregulation of miR-26b in osteosarcoma elevated the levels of CTGF and Smad1, facilitating osteosarcoma metastasis.

References

Feb 14, 2004·The Journal of Investigative Dermatology·Andrew LeaskDavid J Abraham
Jun 10, 2005·Nature·Jun LuTodd R Golub
Jun 21, 2005·Nature Genetics·Isaac BentwichZvi Bentwich
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Nov 30, 2006·Journal of Cell Science·Andrew Leask, David J Abraham
Dec 17, 2008·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Stefan S BielackHeribert Jürgens
Oct 9, 2009·The New England Journal of Medicine·Junfang JiXin Wei Wang
Sep 14, 2010·Journal of Cellular and Molecular Medicine·Yan-Lei MaHuan-Long Qin
Mar 24, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mitsuhiko OsakiTakahiro Ochiya
Jul 16, 2011·PloS One·Sashidhar S NakerakantiMaria Trojanowska
Jul 30, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Raquel UrtasunMatias A Avila
Feb 22, 2012·Cancer Research·Kevin B JonesRami I Aqeilan
Apr 25, 2012·The American Journal of Pathology·Junfeng ZhuLiantang Wang
Jun 12, 2013·Frontiers in Bioscience (Landmark Edition)·Shensheng GuBin Liu
Jan 28, 2014·Biochemical and Biophysical Research Communications·Yong GaoCao Yang
Jun 14, 2014·Advances in Experimental Medicine and Biology·Carol H LinBang H Hoang
Jun 14, 2014·Biochemical and Biophysical Research Communications·Jianwen ZouBingchang Zhang
Oct 9, 2014·Molecular Therapy : the Journal of the American Society of Gene Therapy·Meng XuYan Wang

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Citations

Feb 14, 2016·Biochemical and Biophysical Research Communications·Meng LiHua Du
Feb 2, 2016·Biochemical and Biophysical Research Communications·Dongmei LiKui Liu
Jun 30, 2018·Clinical and Experimental Pharmacology & Physiology·Guang-Jun JiaoHai-Chun Liu
Apr 6, 2019·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·G M VieraM S Brassesco
Nov 17, 2016·Oncotarget·Junli ChangYanping Yang
Apr 6, 2018·International Journal of Molecular Medicine·Jifu ZhengZheng Yuan

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