MicroRNA-275 and its target Vitellogenin-2 are crucial in ovary development and blood digestion of Haemaphysalis longicornis

Parasites & Vectors
Jiawei HaoGuangyuan Liu

Abstract

The hard tick Haemaphysalis longicornis is widely distributed in eastern Asia, New Zealand and Australia and is considered the major vector of Theileria and Babesia, harmful parasites to humans and animals. Female ticks need successful blood meals to complete the life-cycle. Therefore, elucidation of the underlying molecular mechanisms of H. longicornis development and reproduction is considered important for developing control strategies against the tick and tick-borne pathogens. Luciferase assays were used to identify the targets of micro RNA miR-275 in vitro. RNAi of Vitellogenin (Vg) was used in phenotype rescue experiments of ticks with miR-275 inhibition, and these analyses were used to identify the authentic target of miR-275 in vivo. The expression of miR-275 in different tissues and developmental stages of ticks was assessed by real-time PCR. To elucidate the functions of miR-275 in female ticks, we injected a miR-275 antagomir into female ticks and observed the phenotypic changes. Statistical analyses were performed with GraphPad5 using Student's t-test. In this study, we identified Vg-2 as an authentic target of miR-275 both in vitro and in vivo by luciferase assays and phenotype rescue experiments. miR-275 plays the...Continue Reading

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Citations

Sep 13, 2019·Scientific Reports·Meghan E HermanceSaravanan Thangamani
Feb 3, 2019·Parasites & Vectors·Muhammad Irfan MalikJinlin Zhou
Mar 20, 2018·Scientific Reports·Marina Amaral XavierCarlos Termignoni

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Methods Mentioned

BETA
Assay
transfections
antisense oligonucleotides
PCR

Software Mentioned

GraphPad

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