MicroRNA-27a reduces mutant hutingtin aggregation in an in vitro model of Huntington's disease

Biochemical and Biophysical Research Communications
Jae-Jun BanManho Kim

Abstract

Huntington's disease (HD) is a fatal genetic disease caused by abnormal aggregation of mutant huntingtin protein (mHtt). Reduction of mHtt aggregation decreases cell death of the brain and is a promising therapeutic strategy of HD. MicroRNAs are short non-coding nucleotides which modulate various genes and dysregulated in many diseases including HD. MicroRNA miR-27a was reported to be reduced in the brain of R6/2 HD mouse model and modulate multidrug resistance protein-1 (MDR-1). Using subventricular zone-derived neuronal stem cells (NSCs), we used in vitro HD model to test the effect of miR-27a on MDR-1 and mHtt aggregation. R6/2-derived NSCs can be differentiated under condition of growth factor deprivation, and the progression of differentiation leads to a decrease of MDR-1 level and efflux function of cells. Immunocytochemistry result also confirmed that mHtt aggregation was increased with differentiation. We transfected miR-27a in the R6/2-derived differentiated NSCs, and examined phenotype of HD, mHtt aggregation. As a result, miR-27a transfection resulted in reduction of mHtt aggregation in HD cells. In addition, MDR-1, which can transport mHtt, protein level was increased by miR-27a transfection. Conversely, knock-down ...Continue Reading

References

Feb 22, 2001·Journal of Neurochemistry·F C LamP B Reiner
Apr 13, 2004·International Clinical Psychopharmacology·Raphael M BonelliHans P Kapfhammer
Oct 22, 2005·The Journal of Clinical Investigation·John R CirritoDavid M Holtzman
Feb 10, 2006·Human Molecular Genetics·Angela HodgesRuth Luthi-Carter
Oct 31, 2006·Nature Genetics·Eugene BerezikovRonald H A Plasterk
Jan 19, 2007·Nature Reviews. Genetics·Kevin Chen, Nikolaus Rajewsky
Dec 18, 2007·Neurobiology of Disease·Rory JohnsonNoel J Buckley
Jan 2, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amy N PackerBeverly L Davidson
Dec 5, 2009·Digestive Diseases and Sciences·Hongwei ZhangXiushan Zheng
Mar 25, 2010·Drugs·Samuel Frank, Joseph Jankovic
Nov 3, 2010·Experimental Neurology·Soon-Tae LeeJae-Kyu Roh
Mar 10, 2011·Physiological Genomics·Nai-Kui Liu, Xiao-Ming Xu
May 17, 2011·Journal of Experimental & Clinical Cancer Research : CR·Xiaohong ZhaoJianguo Hu
Sep 29, 2011·The EMBO Journal·Athanasios ZovoilisAndre Fischer
Nov 4, 2011·RNA Biology·Mithun SinhaNitai P Bhattarcharyya
Jan 7, 2014·Frontiers in Cellular Neuroscience·Simona MaciottaYvan Torrente
Feb 10, 2016·PloS One·Jae-Jun BanManho Kim

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Citations

Jan 24, 2018·Journal of Neurotrauma·Bao-Shu XieJi-Yao Jiang
Sep 12, 2018·Chinese Medical Journal·Dong LiHong-Yan Deng
Apr 3, 2019·Npj Regenerative Medicine·In Ki ChoAnthony Wing Sang Chan
Apr 9, 2020·Journal of Biomedical Science·Yi-Ying Wu, Hung-Chih Kuo
Jul 18, 2019·Frontiers in Aging Neuroscience·Yolanda Espinosa-ParrillaMarcelo Alarcón
Dec 16, 2019·Neurochemistry International·Mario J BertogliatRaghu Vemuganti
Jul 1, 2019·Biomaterials·Weiyu ZhaoYizhou Dong
Aug 24, 2021·Frontiers in Cellular Neuroscience·Xiaoyu Dong, Shuyan Cong
Aug 28, 2021·International Journal of Molecular Sciences·Amy KimJoana Gil-Mohapel

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