MicroRNA-27a regulates hepatic lipid metabolism and alleviates NAFLD via repressing FAS and SCD1

Scientific Reports
Meiyuan ZhangYan Tang

Abstract

MicroRNAs are implicated as crucial mediators in metabolic diseases including obesity, diabetes, and non-alcoholic fatty liver diseases (NAFLD). Here, we show miR-27a attenuated hepatic de novo lipogenesis and alleviated obesity-initiated NAFLD through inhibiting Fasn and Scd1 in liver. Hepatic levels of miR-27a were significantly augmented in HFD-fed and ob/ob mice. Further studies demonstrated that miR-27a directly interacted with 3' untranslated region (3'-UTR) of hepatic Fasn and Scd1 mRNAs and reduced their expression levels in mice. Adenovirus-mediated overexpression of miR-27a robustly blocked sodium oleate-induced triglyceride (TG) accumulation in mouse primary hepatocytes and reduced liver TG contents in mice via repressing hepatic lipogenesis. Furthermore, ectopic expression of hepatic miR-27a impaired lipid contents of livers and attenuated NAFLD development through suppressing lipogenesis in HCD-fed and ob/ob mice. Together, our results reveal a critical role of miR-27a in lipid homeostasis of liver and pathogenesis of NAFLD.

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Citations

May 26, 2018·Scientific Reports·David J SchultzCarolyn M Klinge
Sep 4, 2019·Scientific Reports·Aida JavidanVenkateswaran Subramanian
Apr 16, 2019·Current Opinion in Lipidology·Leslie R SedgemanKasey C Vickers
Aug 28, 2019·American Journal of Physiology. Endocrinology and Metabolism·Jerad H DumoltTodd C Rideout
Feb 20, 2020·Molecular and Cellular Biology·Abrar A KhanNitish R Mahapatra
Sep 19, 2019·American Journal of Physiology. Endocrinology and Metabolism·Shuyue WangYuxin Li
Dec 17, 2020·Frontiers in Endocrinology·Guan WangGuangming Xiang
Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·William LeonardZhongxiang Fang
Feb 10, 2020·Biochemical and Biophysical Research Communications·Meiyuan ZhangWeirong Lu
Mar 28, 2021·Nature Reviews. Molecular Cell Biology·Pamela Agbu, Richard W Carthew
Aug 27, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Xiao-Fei PengWen-Chu Ye
Feb 1, 2022·Environmental Science and Pollution Research International·Hai Duc NguyenMin-Sun Kim

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Methods Mentioned

BETA
PCR
Flow cytometry
transfection
Assay

Software Mentioned

GraphPad
Targetscan

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