MicroRNA-27b Enhances the Hepatic Regenerative Properties of Adipose-Derived Mesenchymal Stem Cells

Molecular Therapy. Nucleic Acids
Kuang-Den ChenChao-Long Chen

Abstract

Adipose-derived mesenchymal stem cells (ASCs) are readily available multipotent mesenchymal progenitor cells and have become an attractive therapeutic tool for regenerative medicine. We herein investigated the mechanistic role of how miR-27b modulated regenerative capacities of ASCs. Intravenous administration of miR-27b-transfected ASCs (ASCs-miR-27b) was conducted after 70% partial hepatectomy (PH). After PH, rats injected with ASCs-miR-27b had decreased inflammatory cytokines and increased hepatocyte growth factor and other related growth factors. We showed that the nature of ASCs-miR-27b to inhibit hepatic stellate cell activation was dependent upon peroxisome proliferator-activated receptor gamma coactivator-1 alpha (PGC-1α) in vitro. Moreover, expression of miR-27b in ASCs induced heme oxygenase-1 (HO-1), resulting in increased production of ATP, protective cytokines/growth factors, and genes involved in mitochondrial biogenesis in a PGC-1α-dependent manner. RNA sequencing (RNA-Seq) analysis revealed drastic transcriptional changes in livers treated with ASCs-miR-27b after PH. The differentially expressed genes classified into "regeneration," "fibrosis," and "mitochondrial biogenesis" clusters were mainly mitochondrial. T...Continue Reading

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Citations

Jan 18, 2020·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Timucin TanerMark D Stegall
Jul 10, 2019·Stem Cell Research & Therapy·Chenxia HuLanjuan Li
Apr 13, 2021·Frontiers in Immunology·Yves-Marie PersDanièle Noël
Jun 28, 2021·BMC Geriatrics·Chia-Chun KaoWei-Liang Chen

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Datasets Mentioned

BETA
GSE69783

Methods Mentioned

BETA
reverse
transfection
RNA-Seq

Software Mentioned

DESeq
Living Image
Ariadne
Pathway Studio
Strand NGS

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