Apr 21, 2012

microRNA-27b suppresses mouse MSC migration to the liver by targeting SDF-1αin vitro

Biochemical and Biophysical Research Communications
Mu-Han LüShi-Ming Yang

Abstract

The SDF-1/CXCR4 axis is critical for inducing stem cell mobilization into the circulation, for homing stem cells to the site of injury, and for stem cell participation in the regeneration of liver tissue. In this study, we have gained insight into the molecular mechanisms involved in regulating the expression of SDF-1α by miRNAs. Using microarray and bioinformatics approaches, we identified six miRNAs with differential expression in damaged liver tissue (21 days after liver injury) compared to normal C57BL/6 murine liver tissue and further confirmed these observations by qPCR; miR-23a, which was identified by other researchers, was also included for comparative purposes. We found that miR-23a, miR-27a and miR-27b expression was significantly lower in the damaged liver than in the normal liver (p<0.05). We further confirmed that miR-27b could directly interact with the 3'UTR of SDF-1α to suppress SDF-1α protein expression using a luciferase reporter assay and Western blot analysis. In addition, we found that the over-expression of miR-27b significantly reduced the directional migration of primary cultured CRCX4-positive murine mesenchymal stem cells (mMSCs) in vitro using a transwell assay. These results suggest that miR-27b may...Continue Reading

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  • Citations23

Citations

Mentioned in this Paper

Real-Time Polymerase Chain Reaction
Carbon Tetrachloride
Flow Cytometry
Stromal Cell-Derived Factor 1
Abdominal Cavity
Transfection
Tissue Membrane
Untranslated Regions
Transdifferentiation
End Stage Liver Disease

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