MicroRNA-29a-3p Downregulation Causes Gab1 Upregulation to Promote Glioma Cell Proliferation.

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Nai-Yuan ShaoCong Cao

Abstract

Glioma causes significant human mortalities annually. Molecularly-targeted therapy is a focus of glioma research. Grb2-associated binding 1 (Gab1) expression and microRNA-29a-3p ("miR-29a-3p") expression in human glioma cells and tissues were tested by Western blotting assay and qRT-PCR assay. shRNA/siRNA strategy was applied to silence Gab1 in human glioma cells. miR-29a or anti-sense miR-29a construct was transfected to human glioma cells. Cell proliferation was tested by BrdU ELISA assay and cell counting assay. We show that expression of Gab1 was significantly elevated in human glioma tissues and cells, which correlated with downregulation of its putative microRNA: miR-29a-3p. In A172 glioma cells and primary human glioma cells, Gab1 shRNA/siRNA inhibited Akt-Erk activation and cell proliferation. Forced-expression of miR-29a-3p downregulated Gab1, inhibiting glioma cell proliferation, whereas miR-29a-3p was in-effective on cell proliferation in Gab1-silenced A172 cells. Furthermore, introduction of a 3'-untranslated region (3'-UTR) mutant Gab1 (UTR-G160A) blocked miR-29a-3p-induced inhibition on Akt signaling and A172 cell proliferation. miR-29a-3p downregulation leads to Gab1 upregulation to promote glioma cell proliferat...Continue Reading

Citations

Dec 4, 2019·Aging·Li-Na ZhouFang Liu
Sep 29, 2020·OncoTargets and Therapy·Qiang AnBing Yang
Aug 15, 2019·Cell Death & Disease·Xuebang ZhangYuxia Duan
Apr 20, 2019·Biochemical and Biophysical Research Communications·Zheng-Wei LiHai-Hui Qin
Jul 22, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Zhuangzhi WuSiqi Chen

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