MicroRNA-29a Functions as a Tumor Suppressor and Increases Cisplatin Sensitivity by Targeting NRAS in Lung Cancer.

Technology in Cancer Research & Treatment
Xin LiuQingxia Fan

Abstract

MicroRNAs have been reported to play an important role in diverse biological processes and progression of various cancers. MicroRNA-29a has been observed to be downregulated in human lung cancer tissues, but the function of microRNA-29a in lung cancer has not been well investigated. In this study, we demonstrated that the expression levels of microRNA-29a were significantly downregulated in 38 pairs of lung cancer tissues when compared to adjacent normal tissues. Overexpression of microRNA-29a inhibited the activity of cell proliferation and colony formation of lung cancer cells, H1299 and A549. Furthermore, microRNA-29a targeted NRAS proto-oncogene in lung cancer cells. In human clinical specimens, NRAS proto-oncogene was highly expressed in human lung cancer tissues compared to normal tissues. More interestingly, microRNA-29a also sensitizes lung cancer cells to cisplatin (CDDP[Please replace "CDDP" with its expansion in the abstract and also provide expansion for the same in its first occurrence in text, if appropriate.]) via its target, NRAS proto-oncogene. Thus, our results in this study demonstrated that microRNA-29a acted as a tumor suppressor microRNA, which indicated potential application of microRNAs for the treatment...Continue Reading

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Citations

Nov 1, 2018·Journal of Cellular Biochemistry·Hongmei YinXiaobo Hu
Nov 7, 2019·Journal of Cellular Biochemistry·Xuemei JiFangyu Wang
Dec 29, 2020·Journal of Clinical Laboratory Analysis·Qiuting WenGuimei Wang
Nov 10, 2020·Life Sciences·Md Nazim Uddin, Xiaosheng Wang

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Methods Mentioned

BETA
GTP
enzymes
Transfection
protein
electrophoresis
Assay
PCR
flow cytometry
FACS

Software Mentioned

miRanda
GraphPad Prism
TargetScan

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