Abstract
Schistosomiasis is one of the world's major public health problems in terms of morbidity and mortality, causing granulomatous inflammation and cumulative fibrosis. This study explored in vivo and vitro effects of miR-29b-3p in granulomatous liver fibrosis by targeting COL1A1 and COL3A1 in Schistosoma japonicum infection. Thirty male Balb/c mice were assigned to normal control and model (percutaneous infection of cercariae of S. japonicum) groups. NIH-3T3 mouse embryonic fibroblasts were designated into blank, NC, miR-29b-3p mimic, TGF-β1, TGF-β1 + NC, and TGF-β1 + miR-29b-3p mimic groups. HE and Masson staining were employed to observe the pathological changes and collagenous fibrosis. The expression of α-SMA, COL1A1, COL3A1, TIMP-1 was determined by immunohistochemistry. The RT-qPCR, Western blotting and immunofluorescence staining were conducted to determine expression of miR-29b-3p, COL1A1, and COL3A1. CCK-8 assay and flow cytometry were performed to evaluate viability and apoptosis. The relative expression of miR-29b-3p decreased in the model group. The model group showed marked fibrosis in liver tissues. The expression of α-SMA, COL1A1, COL3A1, TIMP-1 was higher in the model group than that in the normal control group. Dua...Continue Reading
References
Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SchäferA M Gressner
Mar 10, 2001·Clinics in Liver Disease·D C Rockey
May 10, 2002·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·T LamireauJ Rosenbaum
May 26, 2007·Parasitology·D ChuJ Shen
May 9, 2008·RNA·Dominic Didiano, Oliver Hobert
Feb 3, 2009·FEBS Letters·Juling JiShuang Mei
Nov 7, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Senthil K VenugopalMark A Zern
Mar 11, 2010·Nature Reviews. Molecular Cell Biology·Masafumi InuiStefano Piccolo
Jan 29, 2011·Investigative Ophthalmology & Visual Science·Coralia LunaPedro Gonzalez
Apr 19, 2011·Best Practice & Research. Clinical Gastroenterology·Ursula E Lee, Scott L Friedman
Mar 27, 2012·Biochemical and Biophysical Research Communications·Baocan WangJiangao Fan
May 7, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun WeiXiaoming Fan
Aug 7, 2013·Experimental Biology and Medicine·Jinghua PengYiyang Hu
Dec 4, 2013·International Journal for Parasitology·Jia-Quan HuangQin Ning
Feb 14, 2014·Parasitology Research·Qi LiaoZhongdao Wu
Apr 2, 2014·Parasites & Vectors·Dandan ZhuLingbo Zhang
Aug 12, 2014·Parasitology·Lan LiQin Ning
Jan 16, 2016·Journal of Clinical Medicine·Katsunori YoshidaKazuichi Okazaki
Mar 5, 2016·International Journal of Molecular Sciences·Sung-Chou LiYing-Hsien Huang
Nov 1, 2016·American Journal of Respiratory and Critical Care Medicine·Lai-Ming YungPaul B Yu
Jun 9, 2016·Cell Biochemistry and Function·Chunli LiangXiaoming Fan
Jun 28, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chang Ho JangSung Hwan Ki
Jul 12, 2016·International Journal of Molecular Sciences·Yuhan ChenJason Chia-Hsien Cheng
Aug 3, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yoshinari MatsumotoYoshiki Murakami
Oct 18, 2016·Cornea·Tetsuya ToyonoAlbert S Jun
Nov 4, 2016·Molecular and Cellular Biochemistry·Yu HuangWeili Gu
Nov 5, 2016·Frontiers in Bioscience (Scholar Edition)·Maria D Castellone, Mikko O Laukkanen
Citations
Feb 7, 2018·Frontiers in Pharmacology·Shuai ZhangJihong Yao
Jul 12, 2018·Journal of Cellular and Molecular Medicine·Jintai LuoZhigang Zhao
Aug 17, 2019·IUBMB Life·Zheng-Yuan XieSheng-Lan Tang
Nov 19, 2019·Environmental Toxicology·Qiong ZhangYingbiao Sun
Apr 6, 2020·Inflammation·Fengjin TanZhongkai Yuan
Jan 20, 2020·Aging·Jian WangYuanlin Song
Jul 8, 2019·Journal of Natural Medicines·Junting GongLi Yang
Jun 2, 2020·Frontiers in Bioengineering and Biotechnology·Kaile ZhangXiaolan Fang
Dec 12, 2018·Journal of Cellular Physiology·Yanwei YinYongli Gao
Apr 23, 2019·Experimental and Therapeutic Medicine·Yinghui ZhangYingbo Zhang
Sep 22, 2019·Biological Research·Wei Cao, Youping Feng
Jan 7, 2020·BMC Medicine·Xiumeng HuaJiangping Song
Jan 1, 2020·Parasites & Vectors·Qianglin ChenQuan Gong
May 30, 2020·Physiological Research·R ZhouW Zhang
Mar 29, 2020·Frontiers in Microbiology·Rong LiuHong Jiang
Jan 17, 2020·JCI Insight·Enrique Fuentes-MatteiRoxana S Redis
May 8, 2020·Journal of Ophthalmology·Hongyang LuoXin Luo
Aug 18, 2020·Cell Death & Disease·Qin PanChun-Hua Fang
Jul 13, 2020·Journal of Biochemistry·Xijun GongFangfang Zhou
Oct 20, 2020·PLoS Pathogens·Hongyan KongJiaquan Huang
Jan 22, 2021·Journal of Ovarian Research·Guidong YaoYingpu Sun
Jan 28, 2021·Nephrology·Rumei LuanXuehong Lu
Jan 22, 2021·Life Sciences·Shen PanJun Xin
Mar 7, 2021·Life Sciences·Chao HeXin Yang
Jan 16, 2021·Tissue Engineering. Part B, Reviews·Ana M Gracioso MartinsDonald O Freytes
May 1, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Zhiyong DuCuifeng Wei
Jul 23, 2021·Scientific Reports·Zheng ZhengQiong Cheng
Aug 22, 2021·Scientific Reports·Jinhang ZhuShengying Qin
Aug 23, 2021·Pathology, Research and Practice·Hui ZhaoJun Yu
Mar 8, 2020·Genomics·Banu BayramMatthew P Abdel