MicroRNA-29b-3p prevents Schistosoma japonicum-induced liver fibrosis by targeting COL1A1 and COL3A1.

Journal of Cellular Biochemistry
Ran TaoQin Ning

Abstract

Schistosomiasis is one of the world's major public health problems in terms of morbidity and mortality, causing granulomatous inflammation and cumulative fibrosis. This study explored in vivo and vitro effects of miR-29b-3p in granulomatous liver fibrosis by targeting COL1A1 and COL3A1 in Schistosoma japonicum infection. Thirty male Balb/c mice were assigned to normal control and model (percutaneous infection of cercariae of S. japonicum) groups. NIH-3T3 mouse embryonic fibroblasts were designated into blank, NC, miR-29b-3p mimic, TGF-β1, TGF-β1 + NC, and TGF-β1 + miR-29b-3p mimic groups. HE and Masson staining were employed to observe the pathological changes and collagenous fibrosis. The expression of α-SMA, COL1A1, COL3A1, TIMP-1 was determined by immunohistochemistry. The RT-qPCR, Western blotting and immunofluorescence staining were conducted to determine expression of miR-29b-3p, COL1A1, and COL3A1. CCK-8 assay and flow cytometry were performed to evaluate viability and apoptosis. The relative expression of miR-29b-3p decreased in the model group. The model group showed marked fibrosis in liver tissues. The expression of α-SMA, COL1A1, COL3A1, TIMP-1 was higher in the model group than that in the normal control group. Dua...Continue Reading

References

Jul 1, 1987·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·S SchäferA M Gressner
May 10, 2002·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·T LamireauJ Rosenbaum
May 9, 2008·RNA·Dominic Didiano, Oliver Hobert
Nov 7, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Senthil K VenugopalMark A Zern
Mar 11, 2010·Nature Reviews. Molecular Cell Biology·Masafumi InuiStefano Piccolo
Jan 29, 2011·Investigative Ophthalmology & Visual Science·Coralia LunaPedro Gonzalez
Apr 19, 2011·Best Practice & Research. Clinical Gastroenterology·Ursula E Lee, Scott L Friedman
Mar 27, 2012·Biochemical and Biophysical Research Communications·Baocan WangJiangao Fan
May 7, 2013·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jun WeiXiaoming Fan
Dec 4, 2013·International Journal for Parasitology·Jia-Quan HuangQin Ning
Nov 1, 2016·American Journal of Respiratory and Critical Care Medicine·Lai-Ming YungPaul B Yu
Jun 28, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Chang Ho JangSung Hwan Ki
Aug 3, 2016·Molecular Therapy : the Journal of the American Society of Gene Therapy·Yoshinari MatsumotoYoshiki Murakami
Nov 4, 2016·Molecular and Cellular Biochemistry·Yu HuangWeili Gu
Nov 5, 2016·Frontiers in Bioscience (Scholar Edition)·Maria D Castellone, Mikko O Laukkanen

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Citations

Apr 23, 2019·Experimental and Therapeutic Medicine·Yinghui ZhangYingbo Zhang
Jan 17, 2020·JCI Insight·Enrique Fuentes-MatteiRoxana S Redis
Jan 16, 2021·Tissue Engineering. Part B, Reviews·Ana M Gracioso MartinsDonald O Freytes
May 1, 2021·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Zhiyong DuCuifeng Wei
Aug 23, 2021·Pathology, Research and Practice·Hui ZhaoJun Yu

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