MicroRNA-302b negatively regulates IL-1β production in response to MSU crystals by targeting IRAK4 and EphA2

Arthritis Research & Therapy
Teng MaXikun Zhou

Abstract

Interleukin-1β (IL-1β) is a pivotal proinflammatory cytokine that is strongly associated with the inflammation of gout. However, the underlying mechanism through which the production of IL-1β is regulated has not been fully elucidated. Our previous work identified that miR-302b had an important immune regulatory role in bacterial lung infections. This study was conducted to evaluate the function of miR-302b on monosodium urate (MSU) crystal-induced inflammation and its mechanism. The expression pattern and the immune-regulatory role of miR-302b were evaluated both in vitro and in vivo. The functional targets of miR-302b were predicted by bioinformatics, and then validated by genetic approaches. In addition, the clinical feature of miR-302b was analyzed using serum samples of patients with gouty arthritis. The extremely high expression of miR-302b was observed in both macrophages and mouse air membranes treated with MSU. Intriguingly, overexpression of miR-302b regulated NF-κB and caspase-1 signaling, leading to significantly attenuate MSU-induced IL-1β. By genetic analysis, miR-302b exhibited inhibitory function on IRAK4 and EphA2 by binding to their 3'-UTR regions. Corporately silencing IRAK4 and EphA2 largely impaired MSU-ind...Continue Reading

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Mar 12, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Yao WangYangang Wang
Apr 10, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina BoxbergerUwe K Zettl
Jun 5, 2020·Pharmaceuticals·Robert M Hughes, Jitka A I Virag
Sep 17, 2019·International Journal of Molecular Medicine·Shangzeng WangLicheng Wei
Sep 18, 2020·Endocrinology·Xue LiChongge You
Mar 3, 2021·International Journal of Rheumatic Diseases·Xiang WangYangang Wang
Jun 1, 2021·Expert Review of Clinical Immunology·Paola GalozziFrancesca Oliviero
Aug 20, 2021·Frontiers in Cell and Developmental Biology·Jia-Ni YuanTing-Ting Zhang

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Methods Mentioned

BETA
ELISA
PMA
PCR
transfection
confocal microscopy
lavages
flow
flow cytometry

Software Mentioned

CFX
SPSS
miRanda
- Pro analyzer
miRDB
TargetScan
Gel

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