MicroRNA-30a-5p promotes replication of porcine circovirus type 2 through enhancing autophagy by targeting 14-3-3

Archives of Virology
Xiaomin WangHongjie Fan

Abstract

Accumulating evidence demonstrates that autophagy and microRNAs (miRNAs) play key roles in regulating virus-host interactions and can restrict or facilitate viral replication. In the present study we examined whether a functional relationship exists between autophagy, miRNA and porcine circovirus type 2 (PCV2) infection, using several approaches. We demonstrated that there was a positive correlation between PCV2 infection and autophagy in 3D4/21 cells and autophagy induced by PCV2 infection triggered PCV2 replication. Four miRNA were selected by real-time PCR and further studied, but only miR-30a-5p mimic had a significant effect on PCV2 replication. Overexpression of miR-30a-5p significantly enhanced PCV2 infection and autophagy in a dose-dependent manner. Blockage of miR-30a-5p significantly decreased PCV2 replication. We provided further evidence that miR-30a-5p regulate the link between PCV2 infection and host immune system. Furthermore, miR-30a-5p targeted and regulated 14-3-3 gene, which is a regulator of autophagy. Flow cytometry data demonstrated that miR-30a-5p promotes cell cycle arrest at the G2 phase to regulate PCV2 replication and autophagy by interacting directly with 14-3-3, but not with the PCV2 genome. These d...Continue Reading

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Citations

Feb 13, 2019·International Journal of Molecular Medicine·Pan TanYoushuo Liu
Oct 11, 2017·Frontiers in Cardiovascular Medicine·Kelsey R BoundsPiyali Chatterjee
Apr 2, 2021·Journal of Cellular and Molecular Medicine·Jiaqi LiuYihong Xiao

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Methods Mentioned

BETA
PCR
flow cytometry
lipidation
transfection
Illumina sequencing

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