MicroRNA-30a as a prognostic factor in urothelial carcinoma of bladder inhibits cellular malignancy by antagonising Notch1.

BJU International
Chao ZhangXin Yao

Abstract

To explore the relation between microRNA-30a (miR-30a) and Notch1, and to evaluate the potential prognostic role of miR-30a in invasive urothelial carcinoma of the bladder (UCB). In all, 50 invasive UCB tissue specimens, along with the adjacent bladder tissue specimens were obtained, and the clinical parameters of the 50 patients were analysed. Bioinformatics analysis was performed and miR-30a was selected as a potential miRNA targeting Notch1, with a luciferase assay performed to verify the binding site between miR-30a and Notch1. Quantitative real-time reverse transcriptase-polymerase chain reaction was used to assess the RNA expressions of miR-30a and Notch1, while Western Blotting and immunohistochemical staining were used to assess the protein expression of Notch1. Finally, cell proliferation, cell cycle, cell migration and invasion assays were used to evaluate the cellular effects of miR-30a and Notch1 on the UCB cell lines T24 and 5637. MiR-30a was downregulated in tumour tissues when compared with adjacent bladder tissues (P < 0.001), negatively correlating with Notch1 messenger RNA (R(2) 0.106, P = 0.021) in invasive UCB, and miR-30a expression further decreased in patients with shorter overall survival and disease-fre...Continue Reading

References

Jul 21, 2001·European Urology·S Brandau, A Böhle
Jul 13, 2004·European Urology·Antonio Lopez-Beltran, Rodolfo Montironi
Feb 3, 2006·Cancer Research·Spyros StylianouKeith Brennan
Jul 20, 2006·Current Cancer Drug Targets·L MieleB J Nickoloff
Aug 18, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Nilay S PatelAdrian L Harris
Jun 15, 2007·Molecular Cell·John TsangAlexander van Oudenaarden
Jan 17, 2008·Nature Reviews. Genetics·Witold FilipowiczNahum Sonenberg
Sep 2, 2008·Oncogene·J DufraineJ Kitajewski
Sep 19, 2009·Nature Reviews. Genetics·Carlo M Croce
Sep 24, 2009·Cancer Research·Yunqing LiRoger Abounader
Dec 4, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Benjamin KefasBenjamin Purow
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
Mar 16, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Sadegh Babashah, Masoud Soleimani
May 24, 2012·Autophagy·Yan YuDaolin Tang
Jun 12, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Chao ZhangXu Zhang
Feb 8, 2013·BJU International·Eran RosenbergOfer Nativ
Aug 6, 2013·Urologic Oncology·Elizabeth A GuancialDavid M Berman
Aug 16, 2013·The Journal of Molecular Diagnostics : JMD·Nadine RatertKlaus Jung
Jul 15, 2015·International Journal of Cancer. Journal International Du Cancer·John J ArcaroliWells A Messersmith

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Citations

Aug 31, 2018·Journal of Oncology·Lin-Hong JiangJin-Hai Tang
Jul 22, 2019·Molecular Diagnosis & Therapy·Qi LiJunling Shi
May 5, 2018·Journal of Clinical Medicine·Tomomi FujiiChiho Ohbayashi

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