MicroRNA-30a suppresses tumor progression by blocking Ras/Raf/MEK/ERK signaling pathway in hepatocellular carcinoma

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Kun ZhouGang Sun

Abstract

Emerging reports suggest microRNAs (miRNAs) play a vital role in the progression of malignant tumors. MiR-30a is downregulated in a variety of cancers and acts as a tumor suppressing gene. However, the molecular mechanisms of miRNA-30a in hepatocellular carcinoma (HCC) are still unclear. Hereby, in this study, we detected that miR-30a expression was significantly down-regulated in both HCC tissues compared with adjacent non-cancerous liver tissues, and we also observed that miR-30a expression was lower in HCC cell lines than that of normal controls. By overexpression of miRNA-30a, we evaluated cell growth with CCK-8 assay and cell apoptosis by flow cytometry. The function of miR-30a on cellular migration in HCC cells was assessed. The potential target genes of miR-30a were analyzed with luciferase activity assay. Our data displayed that miR-30a mimic markedly inhibited HCC cell growth, induced cell apoptosis, and upregulated the expression of apoptotic proteins in HepG2 and MHCC976L cells. We also found that upregulation of miR-30a significantly inhibited HCC cells migration and invasion. Bioinformatics analysis revealed K-Ras was a regulative target gene of miR-30a, and further dual luciferase reporter assay showed that miR-30...Continue Reading

Citations

Mar 15, 2018·Frontiers in Endocrinology·Paola MirraClaudia Miele
Dec 19, 2019·Cancer Metastasis Reviews·Karina Bezerra SalomãoMaría Sol Brassesco
May 18, 2020·International Immunopharmacology·Gholamreza Rezaei DanbaranHamed Mohammadi
Dec 11, 2020·BioMed Research International·Mohamed H YousefAnwar Abdelnaser

Related Concepts

microRNA-30c, human
Liver Carcinoma
Malignant Neoplasm of Liver
Neoplasm Invasiveness
Signal Transduction
Disease Progression
Ras Proteins
MAP Kinase Signaling Pathways
RNA, Small Temporal
Mil-raf Protein Kinases

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