Abstract
Semaphorin3A (Sema3A) is a major inhibitory factor of optic nerve (ON) regeneration post-injury. Many microRNAs (miRNAs) are expressed specifically in the mammalian brain and retina and are dynamically regulated during development, suggesting that this group of miRNAs may be associated with neural development. We found that microRNA-30b (miR-30b) bound to the three prime untranslated region (3' UTR) of Sema3A and inhibited the expression of Sema3A mRNA. The mRNA expression level of miR-30b and the protein expression levels of Sema3A, Neuropilin1 (NRP1), PlexinA1 (PlexA1), phosphorylated p38MAPK (p-p38MAPK), and active caspase-3 were all upregulated in retinas from rats with a damaged ON relative to those with an intact ON. Transfection of cultured retinal ganglion cells (RGCs) with an miR-30b mimic led to decreased levels of Sema3A, NRP1, PlexA1, p-p38MAPK, and active caspase-3 protein expression, as well as axon elongation and reduced levels of apoptosis. These findings provide evidence that miR-30b inhibits Sema3A expression. Decreased Sema3A expression promotes axon outgrowth in RGCs due to reduced levels of Sema3A binding to NRP1 and PlexA1 and simultaneously reduces apoptosis by inhibiting the p38MAPK and caspase-3 pathway...Continue Reading
References
Oct 22, 1993·Cell·Y LuoJ A Raper
Aug 22, 1997·Cell·Z He, M Tessier-Lavigne
Oct 16, 1999·Cell·T TakahashiS M Strittmatter
Oct 12, 2002·The Journal of Biological Chemistry·Anat ShirvanArieh S Solomon
Aug 20, 2003·Neuron·Alexander AntipenkoDimitar B Nikolov
Aug 23, 2003·The Journal of Biological Chemistry·Kaoru KikuchiToru Kimura
Apr 15, 2004·Molecular and Cellular Neurosciences·D BagnardN Thomasset
Jul 24, 2004·RNA·Daniel J LucianoStefan Maas
Dec 17, 2005·Stem Cells·Anna M KrichevskyKenneth S Kosik
Aug 15, 2006·Glia·Anat NitzanArieh S Solomon
Apr 3, 2007·RNA·Annaleen VermeulenScott Baskerville
Aug 29, 2007·Investigative Ophthalmology & Visual Science·Amit AroraDavid A C Simpson
Oct 8, 2009·The Journal of Clinical Investigation·Federica MaioneEnrico Giraudo
Jan 8, 2010·BMC Developmental Biology·Amit AroraDavid A Simpson
May 8, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Shira RosenzweigArieh S Solomon
Aug 25, 2010·Schizophrenia Bulletin·Nikolaos MelliosSchahram Akbarian
Dec 8, 2010·Diabetologia·M M H Al-GayyarA B El-Remessy
Mar 11, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yasir A SyedMark R Kotter
Mar 23, 2011·Brain : a Journal of Neurology·Gabrièle PiatonCatherine Lubetzki
Apr 19, 2011·American Journal of Obstetrics and Gynecology·Pei-Pei SongXu Ma
Jul 12, 2011·Cancer Cell·Avital Gaziel-SovranEva Hernando
Jan 4, 2012·The Journal of Cell Biology·Kathleen ZylbersztejnThierry Galli
Oct 3, 2012·PloS One·Sandrine Le GuillouFabienne Le Provost
Citations
Mar 1, 2016·Progress in Retinal and Eye Research·Claudio RaimondiChristiana Ruhrberg
Apr 3, 2016·Neuroscience Bulletin·Songlin ZhouXiaosong Gu
Sep 20, 2016·Epigenomics·Yi-Lan WengGuo-Li Ming
Dec 24, 2019·Neurocase·Dong-Yun YomRyol Ri
Nov 2, 2017·Oncotarget·Wende LiDonghui Xu
May 28, 2020·ELife·Kanella ProdromidouRebecca Matsas
Sep 26, 2020·Journal of Cellular and Molecular Medicine·Xin WangTianyi Wang
Apr 12, 2019·Frontiers in Cellular Neuroscience·Mengchen YangJianning Zhang
Aug 24, 2019·Frontiers in Molecular Neuroscience·Nitin KhandelwalArvind Kumar
Nov 8, 2016·Neural Plasticity·Ping LiChang-Mei Liu
May 19, 2020·BioMed Research International·Tianqi WuLin Fang
Aug 12, 2017·Frontiers in Molecular Neuroscience·Seshadri RamyaS Thameem Dheen
Oct 22, 2020·Cell Death and Differentiation·Chun-Lei DengCheng-Hu Hu
Feb 19, 2021·Frontiers in Neuroscience·Pei LiJingjing Jiang
May 28, 2021·Cell & Bioscience·Liu Yun-JiaYe Jian
Jul 3, 2021·International Journal of Molecular Sciences·Greta Limoni
Oct 9, 2021·Stem Cell Research & Therapy·Ali Rajool DezfulyHossein Salehi