MicroRNA-30b promotes axon outgrowth of retinal ganglion cells by inhibiting Semaphorin3A expression

Brain Research
F HanJ Ye

Abstract

Semaphorin3A (Sema3A) is a major inhibitory factor of optic nerve (ON) regeneration post-injury. Many microRNAs (miRNAs) are expressed specifically in the mammalian brain and retina and are dynamically regulated during development, suggesting that this group of miRNAs may be associated with neural development. We found that microRNA-30b (miR-30b) bound to the three prime untranslated region (3' UTR) of Sema3A and inhibited the expression of Sema3A mRNA. The mRNA expression level of miR-30b and the protein expression levels of Sema3A, Neuropilin1 (NRP1), PlexinA1 (PlexA1), phosphorylated p38MAPK (p-p38MAPK), and active caspase-3 were all upregulated in retinas from rats with a damaged ON relative to those with an intact ON. Transfection of cultured retinal ganglion cells (RGCs) with an miR-30b mimic led to decreased levels of Sema3A, NRP1, PlexA1, p-p38MAPK, and active caspase-3 protein expression, as well as axon elongation and reduced levels of apoptosis. These findings provide evidence that miR-30b inhibits Sema3A expression. Decreased Sema3A expression promotes axon outgrowth in RGCs due to reduced levels of Sema3A binding to NRP1 and PlexA1 and simultaneously reduces apoptosis by inhibiting the p38MAPK and caspase-3 pathway...Continue Reading

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Citations

Mar 1, 2016·Progress in Retinal and Eye Research·Claudio RaimondiChristiana Ruhrberg
Apr 3, 2016·Neuroscience Bulletin·Songlin ZhouXiaosong Gu
Sep 20, 2016·Epigenomics·Yi-Lan WengGuo-Li Ming
Sep 26, 2020·Journal of Cellular and Molecular Medicine·Xin WangTianyi Wang
Apr 12, 2019·Frontiers in Cellular Neuroscience·Mengchen YangJianning Zhang
Aug 12, 2017·Frontiers in Molecular Neuroscience·Seshadri RamyaS Thameem Dheen
Feb 19, 2021·Frontiers in Neuroscience·Pei LiJingjing Jiang
Jul 3, 2021·International Journal of Molecular Sciences·Greta Limoni

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