MicroRNA-30c attenuates contrast-induced acute kidney injury by suppressing NLRP3 inflammasome.

International Immunopharmacology
Jun XuPing Fu

Abstract

Contrast-induced acute kidney injury (CIAKI) is the third most common cause of hospital-acquired AKI. It has been demonstrated that microRNA-30c (miR-30c) was upregulated in the CIAKI. However, the underlying mechanism remain unclear. The CIAKI was induced in miniature pig. The expression profile of miR-30c in the kidney was evaluated by qPCR. The pathways regulated by miR-30c was identified by qPCR and western blot on renal tubular epithelial cells isolated from miniature pig. Finally, the potential therapeutic application of targeting miR-30c was assessed in the pig model of CIAKI. The miR-30c was up-regulated in miniature pig with CIAKI. The miR-30c suppressed cell apoptosis, expression of NLRP3, the secretion of IL-1β and caspase-1 p10 on renal cells stimulated by iohexol in vitro. In the pig model, miR-30c inhibited the CIAKI development. Our data demonstrated that the miR-30c induced by CIAKI could suppress cell apoptosis and kidney injury via targeting NLRP3. Therefore, targeting miR-30c might be a novel therapeutic candidate for CIAKI treatment and prevention.

Citations

Oct 13, 2020·Renal Failure·Balamurugan PackialakshmiXiaoming Zhou
Sep 6, 2020·Life Sciences·Fangfei ZhangFeng Wang
Apr 30, 2021·Frontiers in Cell and Developmental Biology·Shanshan WeiWenqun Li
Apr 27, 2021·American Journal of Physiology. Renal Physiology·Jianni HuangShougang Zhuang

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