microRNA-32 inhibits the proliferation and invasion of the SGC-7901 gastric cancer cell line in vitro

Oncology Letters
Jianfeng ZhangZhenbiao Mao

Abstract

microRNAs (miRNAs) are a class of endogenously expressed, small non-coding RNAs, which suppress their target mRNAs at the post-transcriptional level. miRNAs play key roles in tumor metastasis. The aim of the present study was to investigate the expression of miRNA-32 (miR-32) on the biological behavior of the human gastric cancer cell line, SGC-7901. SGC-7901 cells were transfected with miR-32-mimic, miR-32-inhibitor and empty plasmid vectors using Lipofectamine™ 2000. The expression of GFP was observed by fluorescent microscopy and miR-32 gene expression was detected by quantitative polymerase chain reaction. The cell counting kit-8 assay was performed to evaluate the effect of miR-32 expression on cell proliferation in vitro. Alterations in the migration and metastatic potential of SGC-7901 cells, prior to and following miR-32 gene transfection, were assayed by cell chemotactic migration and invasion tests. The results of the current study showed that the proliferation rate of the transfected SGC-7901 cells overexpressing miR-32 is reduced and cell chemotactic migration and invasion potentials is markedly reduced following miR-32-mimic transfection (P<0.05). In addition, the results demonstrated that overexpression of miR-32 ...Continue Reading

References

Apr 6, 2000·Journal of the National Cancer Institute·G TamuraS J Meltzer
Oct 27, 2001·Science·M Lagos-QuintanaT Tuschl
Oct 27, 2001·Science·R C Lee, V Ambros
Aug 2, 2003·Nature Reviews. Cancer·Yasuhito Yuasa
Nov 3, 2004·Cancer Research·Hiroyuki MutohKentaro Sugano
Sep 27, 2007·Molecular Cancer·William C S Cho
Oct 10, 2008·Cell Cycle·María José BuenoMarcos Malumbres
Nov 13, 2009·Journal of Digestive Diseases·Zhen Biao MaoJun Ling Yang
Mar 20, 2010·Biochemical Society Transactions·Rita BarrosRaquel Almeida
Jul 9, 2010·CA: a Cancer Journal for Clinicians·Ahmedin JemalElizabeth Ward

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Citations

Jan 31, 2018·Thyroid : Official Journal of the American Thyroid Association·Iman JahanbaniOlusegun Mojiminiyi
Jul 6, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jian-Qiang XuYao-Qi Yang
Dec 5, 2014·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Dafeng ZhangJin Xiao
May 30, 2019·Technology in Cancer Research & Treatment·Yuan ZhangJie Gong
Nov 28, 2020·Oncotarget·Adewale Oluwaseun FadakaMervin Meyer
Aug 30, 2021·Leukemia Research·Zohreh RahimiNazanin Jalilian
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Erfei ChenJin Yang
Jul 1, 2014·Journal of Molecular and Cellular Cardiology·Jacqueline R KempSadashiva S Karnik

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Methods Mentioned

BETA
transfection
PCR
chip

Software Mentioned

miRanda
miRtarget
SPSS
TargetScan

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