MicroRNA-325-3p inhibits cell proliferation and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma by down-regulation of aquaporin 5

Cellular & Molecular Biology Letters
Zhitao ZhangXiangjun Yu

Abstract

Hepatitis B virus (HBV) infection is acknowledged as the main cause of hepatocellular carcinoma (HCC). Moreover, previous studies have revealed that microRNAs (miRNAs) widely participate in regulation of various cellular processes, such as viral replication. Hence, the purpose of this study was to investigate the roles of aquaporin 5 (AQP5) and miR-325-3p in the proliferation and apoptosis of HBV-related HCC cells. AQP5 and miR-325-3p expression in both normal and HBV-HCC tissues or cells (both Huh7-1.3 and HepG2.2.15) was detected using qRT-PCR. AQP5 expression was knocked down in HBV-related Huh7-1.3 and HepG2.2.15 cells using small interfering RNA (siRNA) technology. Down-regulation was confirmed using real-time PCR and Western blot analysis. Effects of AQP5 down-regulation on the proliferation and apoptosis were assessed. Dual luciferase reporter gene assay, Western blot and qRT-PCR were employed to evaluate the effect of miR-325-3p on the luciferase activity and expression of AQP5. Moreover, miR-325-3p mimic-induced changes in cellular proliferation and apoptosis were detected through CCK-8 assay, BrdU assay, flow cytometry analysis and ELISA. In this study, the expression of AQP5 was up-regulated in human HBV-HCC tissue, ...Continue Reading

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Citations

Nov 5, 2019·Journal of Cellular Biochemistry·Yuzhuo WangMin Hu
May 21, 2020·Acta Neurologica Scandinavica·Mattia ZanoniAlberto Gajofatto
Jul 1, 2020·Clinical and Experimental Pharmacology & Physiology·Jiping SunShifeng Yang
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Methods Mentioned

BETA
transfection
PCR
flow cytometry
ELISA
Assay

Software Mentioned

TargetScan
GraphPad Prism
GraphPad

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis