MicroRNA-330 inhibited cell proliferation and enhanced chemosensitivity to 5-fluorouracil in colorectal cancer by directly targeting thymidylate synthase

Oncology Letters
Weidong XuJun Hou

Abstract

Colorectal cancer (CRC) is the third most common cancer in males and the second most common in females, worldwide. Currently, 5-fluorouracil (5-FU)-mediated chemotherapy is the adjuvant treatment for patients with CRC following surgical resection. However, a number of patients with CRC develop 5-FU resistance, which is a major challenge for the effective treatment of cancer. Therefore, it is important to investigate the molecular mechanisms underlying chemoresistance and the therapeutic treatments that may improve the treatment of CRC. The present study demonstrated that microRNA (miR)-330 was significantly downregulated in CRC tissues and cell lines. Ectopic miR-330 expression decreased cell proliferation and enhanced cell chemosensitivity to 5-FU via the cell apoptosis pathway in CRC. In addition, thymidylate synthase (TYMS) was revealed to be a direct target gene of miR-330 in CRC. Knockdown of TYMS inhibited CRC cell proliferation, and enhanced cell chemosensitivity to 5-FU by promoting cell apoptosis. In conclusion, the results of the present study indicated that miR-330 targeted TYMS to inhibit the proliferation and enhance the chemosensitivity of CRC cells to 5-FU by promoting cell apoptosis. The present study provided i...Continue Reading

References

Nov 10, 1998·Clinical and Experimental Pharmacology & Physiology·D M Thomas, J R Zalcberg
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Nov 30, 2002·Expert Opinion on Investigational Drugs·Norman L Lehman
Mar 30, 1957·Nature·C HEIDELBERGERJ SCHEINER
Jan 31, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Sanjay PopatRichard S Houlston
Jun 19, 2004·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P NoordhuisG J Peters
Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Sep 17, 2004·Nature·Victor Ambros
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Dec 4, 2008·Gastroenterology·Steven P O'HaraNicholas F LaRusso
Feb 25, 2010·World Journal of Gastroenterology : WJG·An-Gao XuAi-Hua Gan
Mar 23, 2010·Lancet·David CunninghamNaureen Starling
Mar 26, 2011·Science·Christine L Chaffer, Robert A Weinberg
Apr 29, 2011·Gene Therapy·J A Broderick, P D Zamore
Feb 6, 2013·Nature Reviews. Clinical Oncology·Ernst J KuipersMichael Bretthauer
Apr 10, 2013·Nature Reviews. Gastroenterology & Hepatology·Antoine Brouquet, Bernard Nordlinger
Dec 7, 2013·Clinical Journal of Oncology Nursing·Lesley Andrews
Mar 19, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Apr 24, 2014·International Journal of Oncology·Fei LuChunyan Ji
May 2, 2014·BioMed Research International·Paul H Sugarbaker
Jun 1, 2014·Cancer Biomarkers : Section a of Disease Markers·Julien CogetJean-Christophe Sabourin
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
May 4, 2015·Acta Biochimica Et Biophysica Sinica·Xuzhi LiuBaiquan Yu
Jun 10, 2015·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·Z SunR C Zhao

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Citations

Jun 27, 2019·Journal of Cellular Physiology·Behzad MansooriBehzad Baradaran
Jun 30, 2018·Archives of Toxicology·Georgina HarrisLena Smirnova
Jun 20, 2020·Molecular and Cellular Biochemistry·Qingyan ZhouYongbin Gao
Sep 2, 2018·Environmental Geochemistry and Health·Minghui LiXijin Xu
Oct 3, 2020·World Journal of Gastrointestinal Oncology·Amirsaeed Sabeti AghabozorgiEbrahim Eftekhar
Jan 13, 2021·Pharmaceutics·Paula I EscalanteHéctor R Contreras
Oct 16, 2021·Journal of Gastrointestinal Cancer·Leila KarimiTouraj Asvadi Kermani

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Methods Mentioned

BETA
surgical resection
transfection
flow
FCS

Software Mentioned

TargetScan
SPSS
Image Lab
FCS Express

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