MicroRNA-33b inhibits tumor cell growth and is associated with prognosis in colorectal cancer patients

Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico
W LiaoR Sun

Abstract

To explore the role of miR-33b in colorectal cancer (CRC) and the correlation between its expression and prognosis. The expressions of miR-33b between CRC tissues and normal tissues were measured by real-time PCR. The effects of miR-33b on cell proliferation and cell cycle progression were detected by MTT assay, colony formation assay and flow cytometry. The potential regulations of miR-33b on multiple genes expression were verified by Western blot. Furthermore, the association of miR-33b with CRC clinicopathologic features and prognosis was analyzed by Chi-squared test and Kaplan-Meier tests. MiR-33b was downregulated in CRC compared with normal colorectal samples and miR-33b inhibited tumor cell growth and induced cell cycle arrest. Western blot assays and correlation analysis showed that miR-33b could regulate multiple growth-related genes. Moreover, the expression of miR-33b was associated with TNM stage and tumor size, and CRC patients with high miR-33b expression had a better prognosis. Our data suggest that miR-33b functions as a tumor suppressor gene in CRC through regulating cell proliferation and cell cycle.

References

Nov 21, 1998·Veterinary Pathology·K A Schafer
Aug 2, 2001·Diabetes Technology & Therapeutics·H D StricklerH Yu
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jul 17, 2003·International Journal of Cancer. Journal International Du Cancer·Ling YangYude Chen
Nov 1, 2005·The Lancet Oncology·Joseph J Y SungUNKNOWN Asia Pacific Working Group on Colorectal Cancer
Oct 25, 2006·Nature Reviews. Cancer·George A Calin, Carlo M Croce
Feb 13, 2008·The American Journal of Clinical Nutrition·Edward Giovannucci
May 15, 2010·Science·S Hani Najafi-ShoushtariAnders M Näär
Dec 15, 2010·Nature Reviews. Cancer·Martijn C NawijnAnton Berns
Feb 8, 2011·CA: a Cancer Journal for Clinicians·Ahmedin JemalDavid Forman
May 18, 2011·Proceedings of the National Academy of Sciences of the United States of America·Alberto DávalosCarlos Fernández-Hernando
Jul 12, 2011·Oncogene·M ThomasR K Hartmann
Feb 16, 2012·Cell Cycle·Daniel Cirera-SalinasCarlos Fernández-Hernando
Sep 21, 2013·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Julieann RaderJohn M Maris
Mar 20, 2014·The Journal of Clinical Investigation·Matjaz RokavecHeiko Hermeking
Mar 22, 2014·Molecular and Clinical Oncology·Kazuhito MiyachiHisashi Hisatomi
May 30, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Kai-Min Xiang, Xiao-Rong Li
Sep 13, 2014·Diabetes Care·Wenhui ZhaoGang Hu
Mar 7, 2015·Circulation Journal : Official Journal of the Japanese Circulation Society·Koh OnoTakeshi Kimura

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Citations

Jan 17, 2017·Journal of Cellular Physiology·Abdullah MoridikiaJafar Salimian
Feb 19, 2021·Molecular Biology Reports·Igor Lopes Dos SantosVera Aparecida Saddi

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