MicroRNA-34a: A Key Regulator in the Hallmarks of Renal Cell Carcinoma

Oxidative Medicine and Cellular Longevity
Eman A ToraihAly A M Shaalan

Abstract

Renal cell carcinoma (RCC) incidence has increased over the past two decades. Recent studies reported microRNAs as promising biomarkers for early cancer detection, accurate prognosis, and molecular targets for future treatment. This study aimed to evaluate the expression levels of miR-34a and 11 of its bioinformatically selected target genes and proteins to test their potential dysregulation in RCC. Quantitative real-time PCR for miR-34a and its targets; MET oncogene; gene-regulating apoptosis (TP53INP2 and DFFA); cell proliferation (E2F3); and cell differentiation (SOX2 and TGFB3) as well as immunohistochemical assay for VEGFA, TP53, Bcl2, TGFB1, and Ki67 protein expression have been performed in 85 FFPE RCC tumor specimens. Clinicopathological parameter correlation and in silico network analysis have also implicated. We found RCC tissues displayed significantly higher miR-34a expression level than their corresponding noncancerous tissues, particularly in chromophobic subtype. MET and E2F3 were significantly upregulated, while TP53INP2 and SOX2 were downregulated. ROC analysis showed high diagnostic performance of miR-34a (AUC = 0.854), MET (AUC = 0.765), and E2F3 (AUC = 0.761). The advanced pathological grade was associated w...Continue Reading

References

Jul 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·Y Tsujimoto, C M Croce
Jan 15, 1983·International Journal of Cancer. Journal International Du Cancer·J GerdesH Stein
Oct 1, 1982·The American Journal of Surgical Pathology·S A FuhrmanC Limas
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·J ChenM I Simon
Apr 3, 1998·Mineral and Electrolyte Metabolism·A B Roberts
Jul 27, 1999·International Journal of Cancer. Journal International Du Cancer·M OliveroM F Di Renzo
Feb 1, 2000·Journal of Cellular Physiology·T Scholzen, J Gerdes
Feb 13, 2001·Biochemical and Biophysical Research Communications·S Y SunG S Wu
Nov 22, 2001·Journal of Neuro-oncology·W WickM Weller
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 13, 2002·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·I SkirnisdóttirB Sorbe
Nov 1, 2002·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Harold F Dvorak
Apr 3, 2003·Genes & Development·Ming Jiang, Jo Milner
Jan 13, 2004·Oncogene·Andrew FeberColin S Cooper
Sep 15, 2004·Urological Research·Dionisios MitropoulosConstantinos Dimopoulos
Sep 30, 2004·International Journal of Cancer. Journal International Du Cancer·Ernst LengyelNadia Harbeck
Nov 13, 2004·The Journal of Urology·Jukka P KallioPaula M Martikainen
Mar 5, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Panayiotis A KyzasJohn P A Ioannidis
Mar 16, 2005·Molecular and Cellular Biology·Eros Lazzerini DenchiKristian Helin
Sep 1, 2005·Genes & Development·Jean-Christophe BourdonDavid P Lane
Oct 6, 2005·Journal of Cellular Physiology·Jörn BullwinkelThomas Scholzen
Oct 18, 2005·Current Opinion in Cell Biology·Ute M MollWolfgang Deppert
Dec 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Huiling HeAlbert de la Chapelle
Jan 19, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Javeed IqbalWing C Chan
Feb 28, 2006·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Hyo-Gyoung KangJae Yong Park
Mar 18, 2006·Cell Death and Differentiation·J E Chipuk, D R Green
May 3, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Vesna IvanovićBogomir Dimitrijević
May 16, 2006·Carcinogenesis·Joan Seoane

❮ Previous
Next ❯

Citations

Jul 25, 2019·International Journal of Molecular Sciences·Eun-Ae KimTae-Jin Lee
May 3, 2019·Cancer Control : Journal of the Moffitt Cancer Center·Eman A ToraihManal S Fawzy
Aug 14, 2020·Cancers·Gonçalo Outeiro-PinhoCarmen Jerónimo
Dec 18, 2020·Frontiers in Oncology·Soudeh Ghafouri-FardMohammad Taheri
Feb 20, 2021·Biotechnology and Applied Biochemistry·Jun LiuZhongwei Ren
May 8, 2021·Cancer Metastasis Reviews·Sijing LiLi Sun
Sep 10, 2019·Ecotoxicology and Environmental Safety·Daniela Eliza MarinIonelia Taranu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

mirPath
TarBase
DIANA

Related Concepts

Related Feeds

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Autophagy & Aging: Inhibitors

The feed focuses on the role of nuclear export inhibitors and their effect on autophagy and the aging process.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.