MicroRNA-34a Attenuates Metastasis and Chemoresistance of Bladder Cancer Cells by Targeting the TCF1/LEF1 Axis

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xiaobing LiuLongkun Li

Abstract

Chemoresistance is largely responsible for relapses of bladder cancer during clinical therapy. However, the molecular mechanisms involved in the chemoresistance of bladder cancer are unclear. Growing evidence supports the theory that microRNAs (miRNAs) play an important role in chemotherapeutic drug resistance because they are downregulated in many malignancies that have been implicated in the regulation of diverse processes in cancer cells. More specifically, the extent and precise mechanism of the involvement of miR-34as in chemoresistance to epirubicin (EPI) in the treatment of bladder cancer remains unclear. In this study, real-time quantitative polymerase chain reaction (PCR) was used to analyze the expression of miR-34a in bladder cancer cell line BIU87 and its EPI chemoresistant cell line BIU87/ADR. The miR-34a profiles in bladder cancer tissues were obtained from The Cancer Genome Atlas database. The effect of miR-34a on chemosensitivity was evaluated by cell viability assays, colony formation assays, and in vivo experimentation. Apoptosis and the cell cycle were examined by flow cytometry. A luciferase reporter assay was used to assess the target genes of miR-34a. Western blot and qPCR were used to analyze the expressi...Continue Reading

Citations

Jul 22, 2019·Molecular Diagnosis & Therapy·Qi LiJunling Shi
Feb 18, 2020·Journal of Cellular Physiology·Sanaz NaghizadehBehzad Mansoori
Oct 30, 2020·Cancers·Ilaria CavallariVincenzo Ciminale
Jun 3, 2020·European Journal of Pharmacology·Milad AshrafizadehMasoud Najafi
May 8, 2021·Cancer Metastasis Reviews·Sijing LiLi Sun
May 3, 2020·Anti-cancer Agents in Medicinal Chemistry·Mohamed A RaghebAbdel-Hady A Abdel-Wahab

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