MicroRNA-34a inhibits human trophoblast cell invasion by targeting MYC

BMC Cell Biology
Manni SunTao Meng

Abstract

Preeclampsia, one of the major disorders of pregnancy, is characterized by inadequate trophoblast invasion and defective trophoblast-mediated remodeling of placental vasculature. MicroRNA-34a (miR-34a) has been found to be aberrantly expressed in the placentas of preeclamptic patients, yet its role in placental development and in the pathogenesis of preeclampsia remains elusive. The levels of miR-34a in the placentas of 20 preeclamptic patients and 20 healthy subjects were determined by real time-PCR, and miR-34a was found significantly elevated in the preeclamptic placentas. Further, the function of miR-34a in trophoblast cells was investigated by overexpressing miR-34a in JEG-3 trophoblast cell line. Overexpression of miR-34a in JEG-3 cells inhibited cell proliferation, migration and invasion. In addition, elevated expression of miR-34a reduced the expression of both endogenous and ectopic MYC. Moreover, we identified that MYC mRNA was a direct target of miR-34a in JEG-3 cells by dual luciferase reporter assay, and found that downregulation of MYC expression by miR-34a targeting significantly reduced the invasiveness of JEG-3 cells. Our findings provide preliminary evidence for the diverse functions of miR-34a in trophoblast ...Continue Reading

References

Aug 1, 1994·British Journal of Obstetrics and Gynaecology·J W MeekinsA van Asshe
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Sep 8, 2006·The New England Journal of Medicine·Richard J LevineUNKNOWN CPEP Study Group
Dec 11, 2007·Nature Genetics·Tsung-Cheng ChangJoshua T Mendell
Jan 26, 2008·Clinical Chemistry·Stephen S C ChimY M Dennis Lo
Jan 19, 2010·Annual Review of Pathology·Brett C YoungS Ananth Karumanchi
Jan 26, 2010·Nature Reviews. Immunology·Ryan M O'ConnellDavid Baltimore
Nov 26, 2010·American Journal of Obstetrics and Gynecology·Daniel A EnquobahrieMichelle A Williams
Dec 3, 2010·The Journal of Pathology·Thalia A FaraziThomas Tuschl
Dec 18, 2010·Human Reproduction·R UdayashankarH D Moore
Feb 8, 2011·Oncogene·E SotilloA Thomas-Tikhonenko
Mar 17, 2011·Cancer Research·Anita Wolfer, Sridhar Ramaswamy
Feb 7, 2012·Accounts of Chemical Research·Zhonghan Li, Tariq M Rana
Jun 23, 2012·Placenta·D M Morales-PrietoU R Markert
Oct 20, 2012·Nature Reviews. Drug Discovery·Eva van Rooij, Eric N Olson
Jul 13, 2013·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·James S BetoniGerard Tromp
Oct 2, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Ludivine DoridotDaniel Vaiman
Oct 10, 2013·American Journal of Hypertension·Maria L MirandaPablo Stiefel
Dec 7, 2013·Frontiers in Genetics·Ludivine DoridotDaniel Vaiman
Jan 1, 2014·The Journal of Biological Chemistry·Stephen J RenaudMichael J Soares
Mar 20, 2014·The Journal of Clinical Investigation·Matjaz RokavecHeiko Hermeking

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Citations

Apr 5, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Heyam HayderChun Peng
Nov 2, 2019·Cells·Xiang-Qun Hu, Lubo Zhang
Sep 20, 2019·Journal of Biochemistry·Lianzhi WuJing Yang
Feb 7, 2019·Disease Markers·Agata SakowiczTadeusz Pietrucha
May 28, 2020·International Journal of Molecular Sciences·Asghar AliQuinton A Winger
Jan 23, 2020·International Journal of Molecular Sciences·Dessie Salilew-WondimDawit Tesfaye
Mar 21, 2020·Journal of Biochemistry·Lianzhi WuJing Yang

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Methods Mentioned

BETA
PCR
transfection

Software Mentioned

Gel Pro Analyzer
GraphPad PRISM

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