MicroRNA-34a suppresses invasion and metastatic in esophageal squamous cell carcinoma by regulating CD44

Molecular and Cellular Biochemistry
Jianhui ZuoZaicheng Yu

Abstract

In human esophageal squamous cell carcinoma (ESCC), miR-34a was downregulated and could inhibit in vitro cell proliferation and migration. However, the underlying mechanism was not clear yet. The expression levels of mRNA and protein were detected by quantitative real-time PCR or western blotting, respectively. MiR-34a was knocked down or overexpressed and transfected into human ESCC cell lines ECA109 and TE-13, respectively. Cell migration and wound healing assays were used to examine the effect on migration and invasion in vitro. Animal models were used to examine the role of miR-34a in metastasis in vivo. Luciferase assay was carried out to validate the potential target of miR-34a. CD44 was upregulated and miR-34a was downregulated in ESCC tissues and cell lines. The linear regression analysis showed that CD44 expression was negatively correlated with the level of miR-34a. Luciferase assay showed that miR-34a interacted with a putative binding site in the CD44 3'UTR. MiR-34a was found to negatively regulate the expression of CD44. In vitro experiment showed that miR-34a overexpression inhibited ESCC cell invasion and migration; whereas miR-34a knockdown showed reversed results. MiR-34a also inhibited esophagus tumor growth a...Continue Reading

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Citations

Jan 8, 2020·International Journal of Molecular Sciences·Qingqing FengYan-Dong Wang
Dec 12, 2020·Experimental Hematology & Oncology·Hanxiao XuAiguo Liu
Sep 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Dangze SunChao Ding

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Methods Mentioned

BETA
biopsies
surgical resection
transfection
Assay
PCR
protein assay
xenograft
xenografts

Software Mentioned

GraphPad Prism
GraphPad

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