MicroRNA-361-Mediated Inhibition of HSP90 Expression and EMT in Cervical Cancer Is Counteracted by Oncogenic lncRNA NEAT1.

Cells
Daozhi XuHidemichi Watari

Abstract

Epithelial-mesenchymal transition (EMT) is a key process contributing to cervical cancer (CC) metastasis, and microRNAs (miRNAs) modulate the expression of genes implicated in EMT. However, the accurate role of miR-361 in CC-associated EMT and the mechanisms underlying its function in CC remains largely unknown. The functional roles of miR-361 in CC cells were explored by a series of cell functional assays. Luciferase reporter assays were used to demonstrate the potential interaction between miR-361, HSP90, and long non-coding RNA (lncRNA) NEAT1. We detected a reduction of miR-361 expression in CC tissues compared with normal tissues, and miR-361 overexpression inhibited invasion and EMT phenotypes of CC cells by directly targeting a key EMT activator HSP90. Additionally, we detected significantly higher levels of HSP90 in CC tissues compared with normal tissues, and high expression of HSP90 predicted a poorer prognosis. We further identified NEAT1 as a significantly upregulated lncRNA in CC tissues and high expression of NEAT1 was associated with worse survival in CC patients. NEAT1 directly repressed miR-361 expression and played an oncogenic role in CC cell invasion and sphere formation. Conclusions: These results demonstrat...Continue Reading

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Citations

Dec 29, 2020·Frontiers in Cell and Developmental Biology·Daozhi XuHidemichi Watari
Dec 19, 2020·Translational Oncology·Zarema AlbakovaAlexander M Sapozhnikov
Apr 13, 2021·Frontiers in Molecular Biosciences·Bashdar Mahmud HussenSoudeh Ghafouri-Fard
Aug 3, 2021·BioMed Research International·Mona G AlharbiMosleh M Abomughaid

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Methods Mentioned

BETA
transfections
PCR
Protein Extraction
protein assay
transfection
enzyme-linked immunosorbent assay

Software Mentioned

KM
Plotter
UALCAN
microRNA
ImageJ
Targetscan
SurvExpress
SPSS

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