MicroRNA-3666 inhibits lung cancer cell proliferation, migration, and invasiveness by targeting BPTF

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Linqing PanRanran Tang

Abstract

A previous study by our group indicted that overexpression of bromodomain PHD-finger transcription factor (BPTF) occurs in lung adenocarcinoma, and is closely associated with advanced clinical stage, higher numbers of metastatic lymph nodes, the occurrence of distant metastasis, low histological grade, and poor prognosis. Down-regulation of BPTF inhibited lung adenocarcinoma cell proliferation and promoted lung adenocarcinoma cell apoptosis. The purpose of this study is to identify valuable microRNAs (miRNAs) that target BPTF to modulate lung adenocarcinoma cell proliferation. In our results, we found that miR-3666 was notably reduced in lung adenocarcinoma tissues and cell lines. Using an miR-3666 mimic, we discovered that cell proliferation, migration, and invasiveness were suppressed by miR-3666 overexpression, but these were all enhanced when the expression of miR-3666 was reduced. Moreover, bioinformatics analysis using the TargetScan database and miRanda software suggested a putative target site in BPTF 3'-UTR. Furthermore, using a luciferase reporter assay, we verified that miR-3666 directly targets the 3'-UTR of BPTF. Using Western blot we discovered that overexpression of miR-3666 negatively regulates the protein expre...Continue Reading

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Citations

May 31, 2020·Journal of Clinical Laboratory Analysis·Li-Hong HeYong Meng
Oct 12, 2019·Experimental and Therapeutic Medicine·Ronglin ChenJiefu Lu
Apr 10, 2019·Journal of Cellular Biochemistry·Sheng YangGeyu Liang
Dec 25, 2019·Signal Transduction and Targeted Therapy·Yuan ChengXiawei Wei

Related Concepts

Western Blotting
Down-Regulation
Luciferases
Lymph Nodes
Neoplasm Metastasis
Signal Pathways
Computer Software
Transcription Factor
Cell Line, Tumor
Adenocarcinoma of Lung (Disorder)

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