MicroRNA-3666 inhibits lung cancer cell proliferation, migration, and invasiveness by targeting BPTF

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
Linqing PanRanran Tang


A previous study by our group indicted that overexpression of bromodomain PHD-finger transcription factor (BPTF) occurs in lung adenocarcinoma, and is closely associated with advanced clinical stage, higher numbers of metastatic lymph nodes, the occurrence of distant metastasis, low histological grade, and poor prognosis. Down-regulation of BPTF inhibited lung adenocarcinoma cell proliferation and promoted lung adenocarcinoma cell apoptosis. The purpose of this study is to identify valuable microRNAs (miRNAs) that target BPTF to modulate lung adenocarcinoma cell proliferation. In our results, we found that miR-3666 was notably reduced in lung adenocarcinoma tissues and cell lines. Using an miR-3666 mimic, we discovered that cell proliferation, migration, and invasiveness were suppressed by miR-3666 overexpression, but these were all enhanced when the expression of miR-3666 was reduced. Moreover, bioinformatics analysis using the TargetScan database and miRanda software suggested a putative target site in BPTF 3'-UTR. Furthermore, using a luciferase reporter assay, we verified that miR-3666 directly targets the 3'-UTR of BPTF. Using Western blot we discovered that overexpression of miR-3666 negatively regulates the protein expre...Continue Reading


Feb 22, 2012·EMBO Molecular Medicine·Marilena V Iorio, Carlo M Croce
Jan 22, 2013·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Sep 17, 2013·Nature Methods·Abel Gonzalez-PerezNuria Lopez-Bigas
Oct 15, 2013·Nature Genetics·Cristina Balbás-MartínezFrancisco X Real
Dec 29, 2013·Biochemical and Biophysical Research Communications·Xiao ZhangRui Zhang
Nov 5, 2014·Digestive Diseases and Sciences·Shuai XiaoXianzhou Lu
Jul 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Ri CuiCarlo M Croce
Sep 19, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Lin LiPing Li
Feb 24, 2016·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Ju Hwa LeeSug Hyung Lee
Mar 5, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Gang WangLei Chen
May 23, 2017·Theranostics·Yuanyuan QinChaoshi Niu
Jul 14, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Biying ZhuDaoguang Yan
Aug 11, 2018·Molecular Cancer Research : MCR·Xiaomei SunBeihua Kong


May 31, 2020·Journal of Clinical Laboratory Analysis·Li-Hong HeYong Meng
Oct 12, 2019·Experimental and Therapeutic Medicine·Ronglin ChenJiefu Lu
Apr 10, 2019·Journal of Cellular Biochemistry·Sheng YangGeyu Liang
Dec 25, 2019·Signal Transduction and Targeted Therapy·Yuan ChengXiawei Wei

Related Concepts

Western Blotting
Lymph Nodes
Neoplasm Metastasis
Signal Pathways
Computer Software
Transcription Factor
Cell Line, Tumor
Adenocarcinoma of Lung (Disorder)

Related Feeds


Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Cell Migration in Cancer and Metastasis

Migration of cancer cells into surrounding tissue and the vasculature is an initial step in tumor metastasis. Discover the latest research on cell migration in cancer and metastasis here.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.