MicroRNA-374a Inhibits Aggressive Tumor Biological Behavior in Bladder Carcinoma by Suppressing Wnt/β-Catenin Signaling

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Xiaoliang ChenXinquan Gu

Abstract

microRNA (miR)-374a plays a crucial role in cancer progression by promoting the metastasis and proliferation of various types of malignant tumors. Because its role in bladder cancer is unknown, we investigated whether miR-374a affects the progression of bladder cancer and studied the underlying mechanism. The Cancer Genome Atlas was used to analyze the clinical relevance of miR-374a. Quantitative PCR, western blotting, and luciferase and immunofluorescence assays were used to detect the expression patterns, downstream targets, and function of miR-374a in bladder cancer cells. Apoptosis was evaluated by flow cytometry after cisplatin treatment. Via in silico analysis, low levels of miR-374a were associated with poor prognosis in bladder cancer patients with distant metastasis. WNT5A was a direct target of miR-374a in two bladder cancer cell lines. miR-374a mimic abrogated the metastatic potential and invasiveness of bladder cancer cells via WNT5A downregulation in both T24 and TCCSUP human bladder cancer cells; the opposite was observed with miR-374a inhibitor. In addition, miR-374a treatment reduced the phosphorylation and nuclear translocation of β-catenin. Cisplatin treatment significantly increased the apoptosis rate. Expres...Continue Reading

Citations

Feb 26, 2019·Technology in Cancer Research & Treatment·Ye ZhangHaozhe Piao
May 30, 2019·Cancers·Masahiro Shibata, Mohammad Obaidul Hoque
Nov 21, 2020·Medical Oncology·Payam Kheirmand PariziSeyed-Alireza Esmaeili
Feb 5, 2021·Pathology, Research and Practice·Weijian ZhouChaoying Liu
Dec 20, 2020·Cellular Signalling·Guanlin WuGang Fan
Sep 17, 2020··Elya Khunazatus ShimaSilmy Rufiatin Nisa’

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