MicroRNA 375 modulates hyperglycemia-induced enteric glial cell apoptosis and Diabetes-induced gastrointestinal dysfunction by targeting Pdk1 and repressing PI3K/Akt pathway

Scientific Reports
Yan ChenHuatian Gan

Abstract

Diabetic neuropathy can damage systemic nervous system, including alteration of enteric nervous system and subsequent gastrointestinal dysfunction. The effect of diabetes on enteric glia cell (EGC) is not clear. We investigated the effect of diabetes and hyperglycemia on EGC, and the role of microRNA375 in modulating EGC survival in vivo and in vitro. Streptozotocin-induced diabetic mice were intraperitoneally injected with microRNA375 inhibitor or its negative control. EGC was transfected with microRNA375 inhibitor or its mimic. Diabetes mice with gastrointestinal dysfunction showed increased apoptosis of EGC (no difference in cell numbers) and gene expression of micorRNA375 in the myenteric plexus. Hyperglycemia triggered apoptosis of EGC in vitro with decreased expression of Pdk1 and p-Akt, but increased expression of micorRNA375. MicorRNA375 mimic induced apoptosis of EGC in vitro with repressed Pdk1and p-Akt. MicorRNA375 inhibitor could both prevent hyperglycemia-induced apoptosis of EGC in vitro and diabetes-induced gastrointestinal dysfunction in vivo. Our results suggest that diabetes-induced gastrointestinal dysfunction is related to increased apoptosis of EGC in the myenteric plexus. Hyperglycemia can increase the exp...Continue Reading

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References

Aug 10, 2000·The Journal of Clinical Investigation·C C WatkinsC D Ferris
Apr 27, 2002·Annals of the New York Academy of Sciences·Andrea M VincentJames W Russell
Sep 24, 2002·Arquivos de neuro-psiquiatria·Maria Montserrat D P FurlanMarcílio H de Miranda Neto
Feb 20, 2003·Brain Research. Molecular Brain Research·Fang Cai, Cinda J Helke
Jun 17, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·C GuoJ W Wiley
Jul 19, 2005·Current Pharmaceutical Design·Sho-ichi Yamagishi, Tsutomu Imaizumi
Feb 3, 2006·The Journal of Clinical Investigation·Mallappa AnithaShanthi Srinivasan
May 8, 2007·Laboratory Investigation; a Journal of Technical Methods and Pathology·Gabrio BassottiBruno Salerni
Mar 22, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shi-Yi ZhouChung Owyang
Apr 30, 2008·World Journal of Gastroenterology : WJG·Wen-Cai QiuKai-Xing Ai
Jul 28, 2009·Annual Review of Medicine·Ramiro GarzonCarlo M Croce
Aug 28, 2009·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·F DuS Liu
Jan 1, 2010·Autonomic Neuroscience : Basic & Clinical·Wei LiuRongde Wu
Dec 6, 2012·Clinical Pharmacology and Therapeutics·S P Nana-Sinkam, C M Croce
Jun 4, 2013·Autonomic Neuroscience : Basic & Clinical·Chloe Stenkamp-StrahmOnesmo Balemba
Jun 19, 2013·World Journal of Diabetes·Babu KrishnanJoseph M Pappachan
Sep 26, 2013·Clinical Science·Aaron D McClelland, Phillip Kantharidis
Feb 1, 2014·International Journal of Molecular Medicine·Jian ZhouDechun Li
Oct 12, 2014·Gastroenterology·Michel NeunlistRoberto De Giorgio
Feb 24, 2015·Glia·Juliana de Mattos Coelho-AguiarVivaldo Moura-Neto
Sep 8, 2017·Current Treatment Options in Gastroenterology·Allen A Lee, William L Hasler
Oct 25, 2017·Current Treatment Options in Gastroenterology·Marc S Piper, Richard J Saad
Nov 4, 2017·Digestive Diseases and Sciences·Seiichiro FukuharaHidekazu Suzuki

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Citations

Sep 22, 2019·Digestive Diseases and Sciences·Alexandra SelbyFrank K Friedenberg
Dec 21, 2018·International Journal of Molecular Sciences·Angelo MaffeiDaniela Carnevale
Apr 6, 2021·Frontiers in Endocrinology·Olga Barca-Mayo, Miguel López

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Methods Mentioned

BETA
flow cytometry
Transfection
antisense
transfections
PCR

Related Concepts

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.

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