MicroRNA-378 enhances migration and invasion in cervical cancer by directly targeting autophagy-related protein 12.

Molecular Medicine Reports
Dongmei TanYouzhong Zhang

Abstract

Cervical cancer is the second most common type of cancer among women worldwide and a leading cause of mortality in women. Metastases reduce the overall survival rate in patients with cervical cancer. Thus, it is clinically urgent to investigate the molecular mechanism of cervical cancer metastasis. The aim of the present study was to investigate the mechanism of microRNA (miR)‑378 in the metastasis of cervical cancer. In the present study, miR‑378 expression levels were significantly upregulated in cervical cancer tissues and cervical intraepithelial neoplasia III tissues when compared with normal cervix tissues. Re‑expression of miR‑378 significantly promoted tumor migration and invasion in vitro, and metastasis in vivo, while downregulation of miR‑378 suppressed the effect in vitro. Luciferase reporter assay revealed that autophagy‑related protein 12 (ATG12) was a direct target of miR‑378 and its expression was downregulated by miR‑378. In cervical cancer tissues with lymph node metastasis, miR‑378 was upregulated while ATG12 was downregulated when compared with lymph node negative cases. To the best of our knowledge, the present study is the first to provide evidence that miR‑378 may be associated with ATG12. Collectively, t...Continue Reading

References

Dec 22, 1998·Molecular and Cellular Biology·C V Dang
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 7, 2003·The New England Journal of Medicine·Nubia MuñozUNKNOWN International Agency for Research on Cancer Multicenter Cervical Cancer Study Group
Jun 15, 2007·International Journal of Clinical Oncology·Noriaki Sakuragi
Dec 14, 2007·Proceedings of the National Academy of Sciences of the United States of America·Daniel Y LeeBurton B Yang
Feb 6, 2010·Annals of Surgery·Helen M HeneghanMichael J Kerin
Mar 18, 2010·British Journal of Cancer·M TsujiuraE Otsuji
Jun 4, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Bao Hua LiXing Xie
Nov 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zoltan MetlagelTakanori Otomo
Feb 1, 2014·International Journal of Oncology·Bo-Long YuXiang-Ping Li
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jan 7, 2015·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Jichun MaZhaoqun Deng
Feb 11, 2015·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mulin LiuQingyong Ma
Feb 17, 2015·Nature Cell Biology·Lyndsay MurrowJayanta Debnath
Oct 2, 2015·International Journal of Molecular Sciences·Michal FedorkoOndrej Slaby
Apr 28, 2016·Molecular Oncology·Marco CordaniMassimo Donadelli

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Citations

May 2, 2019·Frontiers in Genetics·Marta GòdiaAlex Clop
Aug 29, 2021·Molecular and Cellular Biochemistry·Tandrima Mitra, Selvakumar Elangovan

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Methods Mentioned

BETA
hysterectomy
transfection
PCR
Protein Assay

Software Mentioned

SPSS
TargetScan
ImageJ

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