MicroRNA-383 inhibits doxorubicin resistance in hepatocellular carcinoma by targeting eukaryotic translation initiation factor 5A2

Journal of Cellular and Molecular Medicine
Chaoyong TuWeilin Wang

Abstract

Drug resistance occurs commonly in cancers, especially in hepatocellular carcinoma (HCC). Accumulating evidence has demonstrated that microRNAs (miRNAs) play a vital role in tumour chemoresistance. However, little is known about the role of miR-383 in HCC chemoresistance. In the present study, RT-PCR and western blotting were used to identify the expression profile of miR-383 and eukaryotic translation initiation factor 5A2 (EIF5A2). The bioinformatics website Targetscan was used to predict the target genes of miR-383. In vitro and in vivo loss- and gain-of-function studies were performed to reveal the effects and potential mechanism of the miR-383/EIF5A2 axis in chemoresistance of HCC cells. The expression level of miR-383 correlated negatively with doxorubicin (Dox) sensitivity. Overexpression of miR-383 promoted HCC cells to undergo Dox-induced cytotoxicity and apoptosis, whereas miR-383 knockdown had the opposite effects. EIF5A2 was predicted as a target gene of miR-383. EIF5A2 knockdown sensitized HCC cells to Dox. Moreover, miR-383 inhibition-mediated HCC Dox resistance could be reversed by silencing EIF5A2. Finally, we demonstrated that miR-383 inhibition could enhance Dox sensitivity by targeting EIF5A2 in vivo. The res...Continue Reading

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Citations

Feb 3, 2021·Autophagy·Soudeh Ghafouri-FardMohammad Taheri
Feb 25, 2021·Pathology, Research and Practice·Soudeh Ghafouri-FardMohammad Taheri
Apr 1, 2021·Journal of Drug Targeting·Mohammad TaheriSoudeh Ghafouri-Fard

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Methods Mentioned

BETA
PCR
Protein Assay
transfection
light microscopy
flow cytometry

Software Mentioned

spss
TargetScan

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