MicroRNA-424-5p inhibits the proliferation, migration, and invasion of nasopharyngeal carcinoma cells by decreasing AKT3 expression

Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas
Chong ZhaoJiping Su

Abstract

This study examined the expression and potential mechanism of microRNA (miRNA)-424-5p in nasopharyngeal carcinoma (NPC). NPC tissues were collected from 40 patients who were enrolled in the study, and skin samples were collected from 26 healthy subjects during plastic surgery as controls. We performed various in vitro assays using miR-424-5p to examine its function in primary NPC-1 cells. Bioinformatics was employed to analyze potential target genes and signaling pathways of miR-424-5p. We found that miR-424-5p expression in NPC tissues is downregulated and negatively correlated with lymph node metastasis and clinical staging. Expression of miR-424-5p in NPC cells was also downregulated, and transfection with miR-424-5p mimics inhibited proliferation, migration, and invasion of NPC-1 cells. Bioinformatics identified the AKT3 gene as a potential target of miR-424-5p and dual luciferase assays confirmed this finding. Upregulation of AKT3 expression rescued the inhibitory effect of miR-424-5p on the proliferation, migration, and invasion. Our results suggest that miR-424-5p inhibited the proliferation, migration, and invasion of NPC cells by decreasing AKT3 expression.

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 27, 2015·International Journal of Molecular Medicine·Wei-Lu WuGan-Di Li
Oct 21, 2016·Journal of Neuro-oncology·Anna JoyBurt G Feuerstein
Jan 10, 2017·Expert Opinion on Therapeutic Targets·SubbaRao V Madhunapantula, Gavin P Robertson
Dec 8, 2017·Journal of Cachexia, Sarcopenia and Muscle·Martin ConnollyPaul R Kemp
Feb 8, 2018·Cancer Research and Treatment : Official Journal of Korean Cancer Association·Ya-Nan JinHao-Yuan Mo
Feb 10, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Peng YeXinghua Long

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Methods Mentioned

BETA
PCR
electrophoresis
Light microscopy
transfection

Software Mentioned

Image Lab
SPSS

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