MicroRNA-449a Inhibition Protects H9C2 Cells Against Hypoxia/Reoxygenation-Induced Injury by Targeting the Notch-1 Signaling Pathway

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Jing ChengFei He

Abstract

The present study aimed to detect the expression of miR-449a and investigate the effect of miR-449a on cell injury in cardiomyocytes subjected to hypoxia/ reoxygenation (H/R) and its underlying mechanisms. The expression of miR-449a was determined using reverse transcription-polymerase chain reaction in both neonatal rat ventricular myocytes and H9C2 cells. For gain-of-function and loss-of-function studies, H9C2 cells were transfected with either miR-449a mimics or miR-449a inhibitor. The target gene of miR-449a was confirmed by a dual-luciferase reporter assay. Apoptosis was analyzed by both flow cytometry using Annexin V and propidium iodide and transferase-mediated deoxyuridine triphosphate-biotin nick end labeling (TUNEL). Necrosis was confirmed by the detection of lactate dehydrogenase release. The cell viability was measured using the methylthiotetrazole method. The protein levels of Notch-1, Notch-1 intracellular domain, hairy and enhancer of split-1 (Hes-1), and apoptosis-related genes were measured by Western blot analysis. MiR-449a was significantly upregulated in both neonatal rat ventricular myocytes and H9C2 cells subjected to H/R. However, H/R-induced cell apoptosis and necrosis were markedly reduced by miR-449a i...Continue Reading

Citations

Dec 16, 2019·Heart Failure Reviews·Abdelrahman Yousry Afify
Jan 25, 2020·Oxidative Medicine and Cellular Longevity·Md Sayed Ali Sheikh
Oct 23, 2019·Molecular Medicine Reports·Wenjuan WeiJian Li
Jan 16, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Ali Mohammad Pourbagher-ShahriSaeed Samargahndian
Sep 4, 2021·Experimental and Therapeutic Medicine·Qiu Qian, Yingxiang Xie
Apr 20, 2019·Experimental and Molecular Pathology·Yang ZhangDejin Min

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