MicroRNA-488 inhibits proliferation, invasion and EMT in osteosarcoma cell lines by targeting aquaporin 3

International Journal of Oncology
Jing QiuZhi Guo

Abstract

It has been reported that aquaporin 3 (AQP3) expression is associated with the progression of numerous types of cancer and microRNA (miRNA/miR) processing. However, the effects and precise mechanisms of AQP3 in osteosarcoma (OS) have not been fully elucidated. The present study aimed to investigate the interaction between AQP3 and miR‑488 in OS. The reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) assay was performed to detect the levels of AQP3 and miR‑488 in OS tissues and cell lines, respectively. Cell proliferation, invasion and epithelial-mesenchymal transition (EMT) were detected to analyze the biological functions of miR‑488 and AQP3 in OS cells. Furthermore, mRNA and protein levels of AQP3 was measured by RT‑qPCR and western blot analysis. Furthermore, AQP3 was validated as an miR‑488 target using luciferase assays in OS cells. The present study revealed that the miR‑488 level was significantly downregulated in OS tissues and cell lines, and that the expression of AQP3 was markedly increased. Notable, the low miR‑488 expression level was associated with upregulated AQP3 expression in OS tissues. Furthermore, introduction of miR‑488 markedly suppressed the proliferation, invasion and EMT of OS cells...Continue Reading

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Citations

Jun 18, 2020·Journal of Clinical Medicine·Araceli García-MartínezAntonio Picó
Apr 3, 2019·International Journal of Molecular Sciences·Mohammed Abir-AwanRoslyn M Bill
Feb 25, 2021·American Journal of Physiology. Cell Physiology·Sarannya EdamanaLene N Nejsum
Jan 29, 2022·Molecular and Cellular Biochemistry·Bernard NsengimanaShaoping Ji

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Methods Mentioned

BETA
transfection
protein assay
ELISA

Related Concepts