Abstract
MicroRNAs (miRNAs) are some RNA molecules that negatively regulate gene expression by binding to the 3'-untranslated region (3'-UTR) of target mRNA molecules. The aim of the study is to investigate the clinical role and functional effects of microRNA-493 (miR-493) in human hepatocellular carcinoma (HCC). Expression of miR-493 in 58 cases of HCC tissues and adjacent normal tissues was determined by using quantitative real-time PCR (qRT-PCR) analyses. In vitro, cell proliferation and invasion capacity was evaluated by CCK8 assay and trans-well invasion assay. Luciferase reporter assay, western blot and qRT-PCR were used to detect the association between miR-493 expression and zinc finger protein X-linked (ZFX) in HCC. Expression of miR-493 was significantly downregulated in HCC tissues compared to adjacent normal tissues. Lower miR-493 expression associated with tumor size, vascular invasion and poor overall survival (OS) and disease free survival (DFS) time of HCC patients. In vitro, transfection of miR-493 mimic in HCC cells inhibited cell proliferation and invasion abilities. However, transfection of miR-493 inhibitor in HCC cells had promoting effects. Luciferase reporter assay, qRT-PCR and western blot analysis demonstrated ...Continue Reading
References
Jun 16, 2007·Gastroenterology·Hashem B El-Serag, K Lenhard Rudolph
Jun 27, 2008·Intervirology·Seung Kew Yoon
Jan 6, 2009·Journal of Cellular and Molecular Medicine·Laura GramantieriMassimo Negrini
Nov 8, 2011·Molecular Cancer Therapeutics·Koji UenoRajvir Dahiya
Mar 4, 2014·International Journal of Cancer. Journal International Du Cancer·Keng Po LaiNathalie Wong
Mar 19, 2014·Oncogene·X ZhouJ-P Yun
Jul 31, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Tingjia CaoXun Cai
Aug 12, 2014·PloS One·Yixue GuZhimin He
Dec 17, 2014·Cancer Letters·Yuan ShiGuosheng Ren
Jan 7, 2015·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Apr 19, 2015·Oncotarget·Guixing JiangLiping Cao
Aug 1, 2015·Cancer Biology & Therapy·Hao WengYingbin Liu
Jan 17, 2016·Biochemical and Biophysical Research Communications·Xiangrui MengQingxia Fan
Mar 2, 2016·International Journal of Molecular Sciences·C Nelson Hayes, Kazuaki Chayama
Mar 12, 2016·Oncotarget·Xuebing YanYuping Gao
Jun 23, 2016·Digestive Diseases·Álvaro Díaz-GonzálezJordi Bruix
Apr 19, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jinli ZhaoLin Chen
Jun 27, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuqiang XuQingshan Huang
Aug 30, 2017·Oncology Reports·Chunlin JiangMing Kuang
Citations
Feb 27, 2019·Genetic Testing and Molecular Biomarkers·Di HuXinguang Qiu
Oct 29, 2019·Current Gene Therapy·Mahmoud ElhefnawiBangly Soliman
Jul 17, 2019·Aging·Chunyong HanJian Yin
Mar 9, 2019·Current Drug Targets·Seyed Mostafa ParizadehAmir Avan
Aug 25, 2020·Medicinal Chemistry Research : an International Journal for Rapid Communications on Design and Mechanisms of Action of Biologically Active Agents·Jian JinRussell A Prough
Mar 25, 2021·Cancer Management and Research·Jie JiangWenzhuo Yang
Apr 2, 2021·Thoracic Cancer·Wei BianShujun Li
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yuelong ZhangQi Zhang