MicroRNA-495 inhibits the high glucose-induced inflammation, differentiation and extracellular matrix accumulation of cardiac fibroblasts through downregulation of NOD1

Cellular & Molecular Biology Letters
Xiaowei WangYanlan Xu

Abstract

MicroRNAs (miRNAs) have physiological and pathophysiological functions that are involved in the regulation of cardiac fibrosis. This study aimed to investigate the effects of miR-495 on high glucose-induced cardiac fibrosis in human cardiac fibroblasts (CFs) and to establish the mechanism underlying these effects. Human CFs were transfected with an miR-495 inhibitor or mimic and incubated with high glucose. The levels of NOD1 and miR-495 were then determined via quantitative RT-PCR. Pro-inflammatory cytokine levels, cell differentiation and extracellular matrix accumulation were respectively detected using ELISA, quantitative RT-PCR and western blot assays. The luciferase reporter assay, quantitative RT-PCR and western blot were used to explore whether NOD1 was a target of miR-495. The effects of miR-495 on the NF-κB and TGF-β1/Smad signaling pathways were also detected via western blot. Our results show that high glucose can significantly increase the expression of NOD1 in a time-dependent manner. Upregulation of miR-495 significantly alleviated the high glucose-induced increases in cell differentiation and collagen accumulation of CFs. Moreover, the bioinformatics analysis predicted that NOD1 was a potential target gene for m...Continue Reading

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Citations

Sep 27, 2018·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Dec 24, 2019·Cellular & Molecular Biology Letters·Rafal Bartoszewski, Aleksander F Sikorski
Apr 3, 2020·The Kaohsiung Journal of Medical Sciences·Jian ZhangYin Liang
Jun 11, 2020·Cell Communication and Signaling : CCS·Fatemeh YousefiMichael R Hamblin
Jul 13, 2018·Journal of Cardiovascular Development and Disease·Tiffany L Dill, Francisco J Naya
Sep 2, 2020·Bioscience, Biotechnology, and Biochemistry·Jingdong GuoXiongxiang Zhu
Mar 9, 2021·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·Jingwen XiaoMeiqin Yu
May 21, 2021·Pediatric Research·Frehiwet T HailuCarmen C Sucharov
Jun 3, 2021·Biomedicines·Victoria Fernández-GarcíaLisardo Boscá

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Methods Mentioned

BETA
transfection
PCR
immunoprecipitation assay
Protein Assay
ELISA

Software Mentioned

GraphPad Prism
Image Lab
GraphPad
TargetScan

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