MicroRNA-497 promotes proliferation and inhibits apoptosis of cardiomyocytes through the downregulation of Mfn2 in a mouse model of myocardial ischemia-reperfusion injury

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Lei QinJie-Yun Liu

Abstract

Myocardial ischemia-reperfusion (I/R) injury affects millions of people worldwide and has a very high mortality rate. Since microRNA-497 (miR-497) has been found to be related with cardiomyocyte apoptosis, this study aimed to explore the effect of miR-497 by targeting Mfn2 in a mouse model of myocardial ischemia-reperfusion (I/R) injury. BALB/c mice were modeled with I/R and some were injected with miR-497 agomir before I/R to observe whether miR-497 alleviates the injury that occurs as a result of I/R. Bioinformatics website and dual-luciferase reporter gene assay were employed in order to detect the relations between miR-497 and Mfn2 gene. Next, cells were extracted to be transfected with different mimic, inhibitor and siRNAs to further explore how miR-497 acts to I/R. Western blot analysis and reverse transcription quantitative polymerase chain reaction (RT-qPCR) were conducted to measure expressions of miR-497, Mfn2, Fas, Bcl-2, Bax and Caspase-3 in myocardial tissues and cardiomyocytes after transfection. CCK-8 assay and flow cytometry were used to determine proliferation, cell cycle distribution and apoptosis of cardiomyocytes in each group after transfection. Mice with I/R had myocardial dysfunction but before the inject...Continue Reading

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Citations

Oct 30, 2019·Cell Death and Differentiation·Youlim HongEun Kyung Lee
Mar 28, 2020·Cardiovascular Research·Luca BragaMauro Giacca
May 17, 2019·DNA and Cell Biology·Wenxi PengDangheng Wei
Sep 19, 2020·Journal of Animal Science and Biotechnology·Shengjie ShiGuiyan Chu
Jan 26, 2021·Journal of Cellular and Molecular Medicine·Xiaoxuan DongJinghai Chen
May 8, 2020·Molecular Therapy. Nucleic Acids·Rong TangZhan-Peng Huang

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