MicroRNA-499 expression distinctively correlates to target genes sox6 and rod1 profiles to resolve the skeletal muscle phenotype in Nile tilapia

PloS One
Pedro G NachtigallDanillo Pinhal

Abstract

A class of small non-coding RNAs, the microRNAs (miRNAs), has been shown to be essential for the regulation of specific cell pathways, including skeletal muscle development, maintenance and homeostasis in vertebrates. However, the relative contribution of miRNAs for determining the red and white muscle cell phenotypes is far from being fully comprehended. To better characterize the role of miRNA in skeletal muscle cell biology, we investigated muscle-specific miRNA (myomiR) signatures in Nile tilapia fish. Quantitative (RT-qPCR) and spatial (FISH) expression analyses revealed a highly differential expression (forty-four-fold) of miR-499 in red skeletal muscle compared to white skeletal muscle, whereas the remaining known myomiRs were equally expressed in both muscle cell types. Detailed examination of the miR-499 targets through bioinformatics led us to the sox6 and rod1 genes, which had low expression in red muscle cells according to RT-qPCR, FISH, and protein immunofluorescence profiling experiments. Interestingly, we verified that the high expression of miR-499 perfectly correlates with a low expression of sox6 and rod1 target genes, as verified by a distinctive predominance of mRNA destabilization and protein translational ...Continue Reading

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Citations

May 12, 2017·Animal : an International Journal of Animal Bioscience·X Y WangJ Yu
Dec 25, 2019·The Journal of Experimental Biology·Bruno Oliveira da Silva DuranDaniel Garcia de la Serrana
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Mar 27, 2021·Fish & Shellfish Immunology·Wei ZhouChao Ran

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Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
MUSCLE
LinRegPCR
TargetScan
Geneious
BiNGO
REST
Cytoscape
RNAhybrid
MEGA

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