MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2.

Journal of Experimental & Clinical Cancer Research : CR
Tian-Song LiangZhang-Suo Liu

Abstract

Epithelial-mesenchymal transition (EMT)-associated proteins play key roles in cancer progression and metastasis with the involvement of microRNAs (miRNAs). This study aims to assess the role of miR-506 working in tandem with LIM Homeobox 2 (LHX2) in EMT and metastasis through the Wnt/β-catenin signaling pathway in nasopharyngeal carcinoma (NPC). Differentially expressed genes associated with NPC were screened using microarray analyses, from which LHX2 was identified. Next, the potential relationship between miR-506 and LHX2 was analyzed. In order to explore the effect of miR-506 or LHX2 on NPC cell proliferation, migration, invasion and apoptosis, serials of mimics, inhibitors or siRNA against LHX2 were transfected into NPC cells. Then, the expression patterns of LHX2, Wnt1, β-catenin, E-cadherin, Vimentin, TCF4 and Twist were determined to assess the influence of miR-506 or LHX2 on EMT as well as the relationship between the Wnt/β-catenin signaling pathway and TCF4. The tumorigenicity and lymph node metastasis (LNM) in xenograft tumors of nude mice were observed. The has-miR-506-3p was identified as the down-regulated gene in NPC based on the microarray data while LHX2 was negatively regulated by miR-506. Over-expression of mi...Continue Reading

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Citations

Nov 17, 2019·Cell Biochemistry and Function·Yongcun LiuLiping Wu
Sep 10, 2020·Cancer Management and Research·Yanping HuXiaomin Zhang
Jan 23, 2021·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·Mingguang ZhouMang Xiao
Oct 24, 2020·Signal Transduction and Targeted Therapy·Yuanbo KangLei Wang
Jan 7, 2021·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Huali XiangLinquan Wu
Apr 28, 2020·Endocrine, Metabolic & Immune Disorders Drug Targets·Xuling LuoZhiwei Chen
Mar 27, 2021·Journal of Experimental & Clinical Cancer Research : CR·Kosuke YoshidaHiroaki Kajiyama
Aug 29, 2020·Endocrine Regulations·Dariia O TsymbalOleksandr H Minchenko
Jul 22, 2021·Biochemistry. Biokhimii︠a︡·Ekaterina V SeminaKseniya A Rubina
Aug 3, 2021·The Kaohsiung Journal of Medical Sciences·Fu-E JinJi-Hai Wang
Aug 3, 2021·Experimental and Therapeutic Medicine·Bingpeng DengYong Wang

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Datasets Mentioned

BETA
GSE64634
GSE53819
GSE12425
GSE13597

Methods Mentioned

BETA
chip
chips
PCR
transfection
immunoprecipitation
xenograft
flow cytometry

Software Mentioned

TargetScan
Quantity One
MAG Biosystems
Gel imaging
Limma
R
pheatmap
Venn
DIANA
SPSS

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