MicroRNA-509-3p increases the sensitivity of epithelial ovarian cancer cells to cisplatin-induced apoptosis

Pharmacogenomics
Wei ChenSongshan Jiang

Abstract

XIAP is upregulated in chemoresistant epithelial ovarian cancer (EOC). However, the molecular mechanism of this dysregulation remains unclear. The regulation of XIAP by miR-509-3p was investigated by luciferase reporter assay, real-time PCR and immunobloting, and the roles of miR-509-3p in cellular proliferation and apoptosis were accessed through MTT and DAPI assays. miR-509-3p, a downregulated miRNA in chemoresistant EOC, can directly target the XIAP via its 3'UTR. Overexpression of miR-509-3p can not only downregulate the expression of XIAP in ovarian cancer cells but also inhibit the proliferation of EOC cells and increase their sensitivity to cisplatin-induced apoptosis. Our data suggest that restoring certain dysregulated miRNAs to their normal levels could increase the therapeutic effects of anticancer drugs.

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Citations

Mar 27, 2021·Journal of Experimental & Clinical Cancer Research : CR·Kosuke YoshidaHiroaki Kajiyama
Oct 2, 2021·Journal of Ovarian Research·Meysam Moghbeli
Oct 29, 2021·Environmental Science and Pollution Research International·Hui WangHeng Wang

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Methods Mentioned

BETA
transfection
antisense oligonucleotides
protein assay
electrophoresis
reverse transcription-PCR
PCR

Software Mentioned

Prism
GraphPad Prism
LightCycler 480
miRanda

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis