MicroRNA-519d-3p inhibits cell proliferation and migration by targeting TROAP in colorectal cancer

Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie
Xiaoyong Ye, Huizeng Lv

Abstract

Increasing evidence suggests that miR-519d-3p functions as tumor suppressor in several tumors, including breast cancer. However, its biological role in the development of colorectal cancer (CRC) still remains unclear. In this study, we found that miR-519d-3p expression level was remarkably down-regulated in CRC tissues samples and cell lines when compared to adjacent normal tissues and cell line by using qRT-PCR detection. Lower miR-519d-3p expression was significantly correlated with TNM stage, tumor size and lymph node metastasis. CRC patients with high level of miR-519d-3p had higher five-year survival rate than those with low expression of miR-519d-3p (p = 0.01178) using Kaplan-Meier analysis. Moreover, multivariate analysis suggested that miR-519d-3p expression might be an independent prognostic indicator for the survival of CRC patients. The in vitro functional analysis, including MTT, flow cytometry and transwell assays indicated that miR-519d-3p overexpression significantly suppressed cell proliferation, migration and invasion, induced cell cycle G0/G1 phase arrest and cell apoptosis of CRC cells. Furthermore, bioinformatics and luciferase reporter assays verified that trophinin associated protein (TROAP) was a direct t...Continue Reading

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Citations

Jan 19, 2019·Oncology Letters·Qiuliang LiuPengliang Wang
Oct 30, 2019·Bioscience, Biotechnology, and Biochemistry·Lishan Ma, Jin Li
Jan 5, 2019·Journal of Cardiovascular Translational Research·Dongying ZhangXiwen Zhang
Jan 28, 2021·Cell Death & Disease·Lei LiXin-Yuan Guan
Mar 29, 2021·Experimental and Molecular Pathology·Min GuManling Tang
May 29, 2021·Journal of Translational Medicine·Shan HuBo Zhao
Jun 18, 2021·Neoplasia : an International Journal for Oncology Research·Baihui LiLili Yang
Sep 9, 2021·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Pihong LiMingdong Lu
Sep 30, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Qiang ZhenJiabao Liu

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