Abstract
Increasing evidence suggests that miR-519d-3p functions as tumor suppressor in several tumors, including breast cancer. However, its biological role in the development of colorectal cancer (CRC) still remains unclear. In this study, we found that miR-519d-3p expression level was remarkably down-regulated in CRC tissues samples and cell lines when compared to adjacent normal tissues and cell line by using qRT-PCR detection. Lower miR-519d-3p expression was significantly correlated with TNM stage, tumor size and lymph node metastasis. CRC patients with high level of miR-519d-3p had higher five-year survival rate than those with low expression of miR-519d-3p (p = 0.01178) using Kaplan-Meier analysis. Moreover, multivariate analysis suggested that miR-519d-3p expression might be an independent prognostic indicator for the survival of CRC patients. The in vitro functional analysis, including MTT, flow cytometry and transwell assays indicated that miR-519d-3p overexpression significantly suppressed cell proliferation, migration and invasion, induced cell cycle G0/G1 phase arrest and cell apoptosis of CRC cells. Furthermore, bioinformatics and luciferase reporter assays verified that trophinin associated protein (TROAP) was a direct t...Continue Reading
References
May 15, 1995·Genes & Development·M N FukudaS Nozawa
Jun 7, 2002·The Biochemical Journal·Daita NadanoMichiko N Fukuda
Mar 16, 2005·Cell·Steven M JohnsonFrank J Slack
Aug 26, 2006·Cancer·Iris VogelaarAnn G Zauber
Mar 16, 2007·Neoplasia : an International Journal for Oncology Research·Daniel R RhodesArul M Chinnaiyan
Jul 7, 2007·Genome Biology·Sergio KaiserBruce J Aronow
Jul 21, 2007·International Journal of Cancer. Journal International Du Cancer·Dong Hyuk KiSun Young Rha
Oct 16, 2007·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D L ZanetteM A Zago
Jan 4, 2008·Molecular Cancer Research : MCR·Jacob Sabates-BellverGiancarlo Marra
Jan 26, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shuo YangJiawei Zhou
Jun 4, 2008·Cancer Research·Minoru ToyotaTakashi Tokino
Dec 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Kotb AbdelmohsenMyriam Gorospe
Dec 10, 2009·Cancer·Brenda K EdwardsLynn A G Ries
Feb 10, 2010·Clinical & Experimental Metastasis·Yi HongPeh Yean Cheah
Mar 23, 2010·Cell Cycle·Kotb AbdelmohsenMyriam Gorospe
Aug 21, 2010·Genes, Chromosomes & Cancer·Jochen GaedckeB Michael Ghadimi
Oct 20, 2010·PloS One·Magdalena SkrzypczakJerzy Ostrowsk
Jun 30, 2011·RNA Biology·Regalla KumarswamyThomas Thum
Jan 21, 2012·The Journal of Pathology·Francesca FornariLaura Gramantieri
Oct 16, 2012·Drug Discovery Today·Diane M PereiraCecília M P Rodrigues
May 3, 2014·OncoTargets and Therapy·Yingxin PangPeishu Liu
Jul 2, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Gang LiuWen Wang
Mar 25, 2015·PloS One·Jie DingXiaoming Zhu
Aug 5, 2015·Oncology Reports·Xin DengBaosheng Wang
Sep 24, 2015·Biosensors & Bioelectronics·Mostafa AzimzadehHossein Naderi-Manesh
Mar 2, 2017·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Nov 10, 2017·Oncology Research·Yifan LianYuehua Huang
Citations
Oct 5, 2018·Digestive Diseases and Sciences·Yan JiaoYahui Liu
Jul 1, 2020·Thoracic Cancer·Yancheng YeZhengguo Li
Jan 19, 2019·Oncology Letters·Qiuliang LiuPengliang Wang
Mar 15, 2020·Aging·Aili WangZhenyang Xu
Oct 30, 2019·Bioscience, Biotechnology, and Biochemistry·Lishan Ma, Jin Li
Jan 7, 2020·Journal of Cellular Biochemistry·Huanxia SunLiming Xu
Aug 17, 2020·Cell Death & Disease·Xiaolong TangHui Qu
Apr 9, 2019·Molecular Medicine Reports·Qing LuoJinbo Liu
Jan 5, 2019·Journal of Cardiovascular Translational Research·Dongying ZhangXiwen Zhang
Sep 29, 2019·Journal of Cellular and Molecular Medicine·Yangyang YaoJun Deng
Jul 4, 2020·Stem Cell Research & Therapy·Baoping DengWeiping Deng
Apr 21, 2020·Frontiers in Neurology·Ye TaoHaiyan Zhang
Oct 12, 2018·Journal of Cellular Biochemistry·Xiling ZhengMin Xu
Mar 20, 2019·Cellular Oncology (Dordrecht)·Jianbing HuangJie He
Jan 28, 2021·Cell Death & Disease·Lei LiXin-Yuan Guan
Oct 30, 2020·OncoTargets and Therapy·Guangzhao ZhangJiahui Du
Dec 31, 2020·Cancer Management and Research·Rui ChenPinlu Jiang
Mar 29, 2021·Experimental and Molecular Pathology·Min GuManling Tang
May 29, 2021·Journal of Translational Medicine·Shan HuBo Zhao
Jun 3, 2021·CNS Neuroscience & Therapeutics·Zong-Qing ZhaoFan Feng
Jun 18, 2021·Neoplasia : an International Journal for Oncology Research·Baihui LiLili Yang
Jul 22, 2021·Cell Cycle·Bin GaoYifei Liu
Sep 9, 2021·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·Pihong LiMingdong Lu
Sep 30, 2021·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Qiang ZhenJiabao Liu
Sep 30, 2021·Cell Cycle·Yanyan ChenHui Xu